Phenotypes Associated with This Genotype

Genotype
MGI:3618376

• Allelic Composition: Mapk8ip1^tm1Rjd/Mapk8ip1^tm1Rjd
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased susceptibility to neuronal excitotoxicity ( J:77616 )

• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate

• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival

• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression

• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice

• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin

abnormal NMDA-mediated synaptic currents ( J:125318 )

• NMDA-mediated current amplitude is decreased relative to in wild-type mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:77616 )

N • contrary to expectations, homozygotes display normal islet morphology, normal expression of insulin and glucagon, as well as normal blood glucose levels and performance in glucose tolerance tests relative to wild-type mice

decreased susceptibility to neuronal excitotoxicity ( J:77616 )

• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate

• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival

• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression

• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice

• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin

cellular

decreased susceptibility to neuronal excitotoxicity ( J:77616 )

• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate

• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival

• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression

• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice

• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin