Phenotypes for Cd19 Prdm1 MGI:3606173 MGI Mouse

abnormal B cell differentiation ( J:101890 )

• following immunization a severe decrease in the number of antigen-specific IgM secreting cells is seen compared to wild-type mice

• a larger increase in CD138-B220+NP+ B cells expressing GL7 is seen at 7 and 14 days after immunization

abnormal plasma cell differentiation ( J:101890 )

• the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants

abnormal memory B cell number ( J:101890 )

• pre-plasma memory B-cells are reduced by 75% in the spleen and 95% in the bone marrow, and CD138-B220+NP+ cells comprise the majority of memory B cells

abnormal plasma cell morphology ( J:101890 , J:114881 )

• antibody stimulating cell development is completely inhibited in cultures of stimulated B cells, however stimulation of B cells with LPS or LPS and IL-4 results in increased class switch recombination (J:114881)

decreased plasma cell number ( J:101890 )

• very few CD138+B220+/- plasma cells are seen at any time before or after immunization

• 5 days after re-challenge a 99% reduction in CD138+ B220+/- NP+ plasma cells is seen

increased spleen germinal center number ( J:101890 )

• more numerous germinal centers are seen after immunization

increased spleen germinal center size ( J:101890 )

• larger germinal centers are seen after immunization

decreased immunoglobulin level ( J:101890 )

• serum Ig levels are reduced in unimmunized mice

decreased IgG level ( J:101890 )

• in mice immunized with NP-Ficoll serum IgG1 and IgG2a levels are significantly lower in mutants compared to wild-type mice and mutant mice fail to secrete Ig in a recall response

decreased IgM level ( J:101890 )

• in mice immunized with NP-Ficoll serum IgM levels are significantly lower in mutants compared to wild-type mice and homozygous mice fail to secrete Ig in a recall response