Analysis Tools
nervous system
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• injection of misfolded preformed fibrils (PFF) of alpha synuclein into the dorsal striatum results in the preservation of dopaminergic neurons in mutant mice as compared to loss of neurons in similarly injected wild type mice
• treatment of neuronal cell cultures from mutant mice with alpha-syn PFF results in less cell death as compared to treated wild type cultures
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• presence of P-alpha synuclein in mutant mice is 50% less at both 30 and 180 days post PFF injection as compared to wild type
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behavior/neurological
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• expected clasping behavior is observed in tail suspension test in PFF-injected mutant mice and PBS-injected mice, however, clasping behavior is abnormal in PFF-injected wild type mice
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• no loss in grip strength is observed in PFF-injected mutant mice, however, PFF-injected wild type mice exhibit reduced forelimb and hindlimb grip strength
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immune system
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• develop splenomegaly after injection with Staphylococcal enterotoxin B
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• slightly increase numbers after Sendai virus infection
• response to chronic viral infection peaked after 15 days and remained about 2X controls through day 62
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• 2 fold increase seen in the spleen after Sendai virus infection
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• 30 days after Sendai virus infection, there is a 2-3 fold increase in the numbers of CD4+ and CD8+ T cells
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• activity reduced
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• greater T cell response than in controls although peak response may be delayed by a day
• subsequent declines in T cell number are also delayed and declines are less than in controls
• after Staphylococcal enterotoxin injection, more T cells are found in S phase and remain there longer
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hematopoietic system
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• develop splenomegaly after injection with Staphylococcal enterotoxin B
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• slightly increase numbers after Sendai virus infection
• response to chronic viral infection peaked after 15 days and remained about 2X controls through day 62
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• 2 fold increase seen in the spleen after Sendai virus infection
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• 30 days after Sendai virus infection, there is a 2-3 fold increase in the numbers of CD4+ and CD8+ T cells
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• activity reduced
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• greater T cell response than in controls although peak response may be delayed by a day
• subsequent declines in T cell number are also delayed and declines are less than in controls
• after Staphylococcal enterotoxin injection, more T cells are found in S phase and remain there longer
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homeostasis/metabolism
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• dopamine levels (and metabolites) are not reduced in mutant mice injected with misfolded preformed fibrils (PFF) of alpha synuclein in contrast to reduced dopamine levels in PFF-injected wild type mice
• behavior in pole test, a measure of dopaminergic function, is preserved in PFF-injected mutant mice, however, PFF-injected wild type mice exhibit impaired behavior
behavior in pole test, a measure of dopaminergic function, is preserved in PFF-injected mutant mice, however, PFF-injected wild type mice exhibit impaired behavior
behavior in pole test, a measure of dopaminergic function, is preserved in PFF-injected mutant mice, however, PFF-injected wild type mice exhibit impaired behavior
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neoplasm
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• slower tumor growth following inoculation with syngeneic murine MC38 colon cancer cells compared to wild-type controls
• approximately 50% of mice are tumor free beyond 200 days after inoculation
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growth/size/body
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• develop splenomegaly after injection with Staphylococcal enterotoxin B
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