Analysis Tools
mortality/aging
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• complete embryonic lethality is seen in female mice with maternal inheritance of the mutant allele and in hemizygous mutant males thus female heterozygotes can only be generated using male chimeras
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• complete embryonic lethality is seen in female mice with maternal inheritance of the mutant allele and in hemizygous mutant males thus female heterozygotes can only be generated using male chimeras
• no live mutants are found beyond E11.5
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cardiovascular system
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• at E11.5 there is an almost complete absence of fetal blood vessels in the labyrinth and chorionic plate
• the primitive vascular plexis forms on the yolk sac but is not remodeled into an organized vascular network
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embryo
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• by E7.5
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• at E7.5 the ectoplacental cone is smaller compared to wild-type
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• at E11.5 the labyrinthine layer is thinner than normal and the demarcation between the labyrinthine and spongiotrophoblast layers is disrupted
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• at E7.5 the chorionic placenta is smaller compared to wild-type
• at E11.5 mutant placentas are smaller compared to wild-type
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• the chorionic trophoblast remains densely packed at the base of the placenta at E7.5 and contains more giant cells compared to wild-type mice
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• at E11.5 the spongiotrophoblast layer is thinner than normal however the giant cell population is expanded and may form multiple cell layers unlike in wild-type mice
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• the mesoderm and endoderm tend to separate resulting in a rough appearing yolk sac in mutants
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• the number of blood islands is reduced in the yolk sac of mutant mice
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• the allantois fails to invade the chorionic plate
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• the primitive vascular plexis forms on the yolk sac but is not remodeled into an organized vascular network
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growth/size/body
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• by E7.5
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vision/eye
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• in F1 females the number of photoreceptor cell bodies in the outer nuclear layer is reduced resulting in an irregular border between the outer nuclear layer and the outer plexiform layer
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nervous system
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• in F1 females the number of photoreceptor cell bodies in the outer nuclear layer is reduced resulting in an irregular border between the outer nuclear layer and the outer plexiform layer
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