Phenotypes Associated with This Genotype

Genotype
MGI:3038603

• Allelic Composition: Grin1^tm2Stl/Grin1^tm2Stl; Tg(Actb-tTA)1Jzt/0; Tg(Camk2a-cre)T29-1Stl/0; Tg(tetO-Grin1/GFP)1Jzt/0
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal learning/memory/conditioning ( J:77659 )

• deficits in learning, memory and conditioning are seen in doxycycline treated mutants where expression of Grin1 is knocked out and expression of Grin1-GFP in the CA1 region of the hippocampus is blocked but deficits in learning, memory and conditioning are not seen in untreated mutants

abnormal contextual conditioning behavior ( J:77659 , J:88689 )

• with doxycycline treatment prior to or immediately after training mutants show significantly fewer freezing responses compared to untreated mutants in a retention test following a fear-conditioning task (J:77659)

• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in a 1-month retention test following a fear-conditioning task is seen (J:77659)

• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:77659)

• with doxycycline treatment for 30 days in the seventh month after training mutants show severe deficits in retention of remote contextual fear memory in a 9-month contextual retention test (J:88689)

• no deficit was seen with doxycycline treatment for 7 days in the seventh month after training (J:88689)

• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:88689)

• 2 months after stopping doxycycline treatment mutants perform normally in 1-day contextual fear retention, visual memory, open field behavior and rotorod tests (J:88689)

abnormal spatial learning ( J:77659 )

• with doxycycline treatment prior to or immediately after training mutants exhibited longer escape latency in a hidden-platform water maze compared to untreated mutants

• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in escape latency is seen

• deficits in spatial learning and memory are also seen in mutants with doxycycline treatment prior to testing in the transfer test compared to untreated mutants

nervous system

absence of NMDA-mediated synaptic currents ( J:77659 , J:88689 )

• with doxycycline treatment excitation postsynaptic potentials are absent in the CA1 hippocampal region (J:77659)

• no long-term potentiation is observed in doxycycline treated homozygotes (J:77659)

• with a 5 day doxycycline treatment no long-term potentiation is observed in mutants (J:88689)