Phenotypes Associated with This Genotype

Genotype
MGI:3036555

• Allelic Composition: Runx1^tm1Soga/Runx1^tm1.1Soga; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:88511 )

N • mutants injected with pIpC to induce expression of Cre recombinase exhibit normal myeloid cell development, neutrophil counts, hemoglobin levels, and maintenance of hematopoietic stem cells in adults

abnormal definitive hematopoiesis ( J:88511 )

abnormal common myeloid progenitor cell morphology ( J:88511 )

• pIpC treated mutants exhibit an increase in apoptosis of myeloid colony-forming cells

abnormal B cell differentiation ( J:88511 )

abnormal T cell differentiation ( J:88511 )

• pIpC treated mutants exhibit a significant block in the maturation of T-cell progenitors at the transition from CD44+CD25+ (double negative 2) to CD44-CD25+ (double negative 3), indicating the accumulation of immature T-cell precursors in the thymus

abnormal megakaryocyte morphology ( J:88511 )

• the immature megakaryocytes observed in bone marrow of mutants injected with pIpC exhibit poorly demarcated membranes and a lower level of polyploidy than controls

abnormal megakaryocyte differentiation ( J:88511 )

• after injection of pIpC to induce expression of Cre recombinase, bone marrow exhibits an arrest in megakaryocytic maturation

abnormal megakaryocyte progenitor cell morphology ( J:88511 )

• after injection of pIpC to induce expression of Cre recombinase, mutants exhibit an increase in hematopoietic progenitor cells

• bone marrow cells from pIpC injected mutants form more megakaryocytic (CFU-Meg), myeloid, and mixed (myeloid and erythroid) colonies in vitro than control bone marrow cells

thrombocytopenia ( J:88511 )

• immediately after injection of pIpC to induce expression of Cre recombinase, mice show a decline in platelet counts to 1/3 to 1/6 of those for the control

immune system

abnormal B cell differentiation ( J:88511 )

abnormal T cell differentiation ( J:88511 )

• pIpC treated mutants exhibit a significant block in the maturation of T-cell progenitors at the transition from CD44+CD25+ (double negative 2) to CD44-CD25+ (double negative 3), indicating the accumulation of immature T-cell precursors in the thymus

cellular

abnormal megakaryocyte differentiation ( J:88511 )

• after injection of pIpC to induce expression of Cre recombinase, bone marrow exhibits an arrest in megakaryocytic maturation