Phenotypes Associated with This Genotype

Genotype
MGI:3033860

• Allelic Composition: Cd276^tm1Mak/Cd276^tm1Mak
• Genetic Background: C.129P2-Cd276^tm1Mak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T-helper 1 physiology ( J:84973 )

• homozygotes develop more severe T helper 1 (TH1)-mediated lung inflammation than wild-type controls

increased interferon-gamma secretion ( J:84973 )

• homozygotes sensitized in T_H1 conditions show a 6-fold increase of IFN-gamma levels in BAL fluid relative to wild-type controls

• when restimulated in vitro with OVA, pulmonary lymph node cell cultures derived from mutant mice sensitized in T_H1 conditions produce ~3 times more IFN-gamma than wild-type cultures, indicating enhanced T_H1 effector activity

increased inflammatory response ( J:84973 )

• upon OVA sensitization, homozygotes develop more severe airway inflammation than wild-type controls in T helper 1 (T_H1) polarizing conditions (GM-CSF plus IL-12), as shown by a 3-fold increase in the number of activated (CD69⁺) CD4 and CD8 T cells in lung, a 6-fold rise in IFN-gamma levels in BAL fluid, and a 3-fold increase in IFN-gamma levels in pulmonary lymph node cell cultures from mice sensitized in T_H1 conditions

hematopoietic system

abnormal T-helper 1 physiology ( J:84973 )

• homozygotes develop more severe T helper 1 (TH1)-mediated lung inflammation than wild-type controls