Phenotypes Associated with This Genotype

Genotype
MGI:2667161

• Allelic Composition: Drap1^tm1Mms/Drap1^tm1Mms
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:80667 )

• no viable homozygotes are recovered from the progeny of heterozygous intercrosses

• however, homozygotes are recovered at normal Mendelian ratios with no detectable abnormalities up to E6.5

embryo

abnormal gastrulation ( J:80667 )

• at E7.0, homozygotes show severe gastrulation defects, including a caudal bulge that corresponds to a significantly expanded primitive streak

• gastrulation defects are associated with increased expression of Nodal and can be partially suppressed by Nodal heterozygosity

abnormal gastrulation movements ( J:80667 )

• at E7.0, homozygotes display little or no mesoderm migration circumferentially to the rostral side, with only limited movement observed anteriorly or extraembryonically

abnormal mesoderm development ( J:80667 )

• at E7.5, homozygotes display absence of mesoderm on the rostral side

• analysis of markers of nascent mesoderm indicates altered regional specification resulting in an expansion of axial mesendoderm at the expense of paraxial, lateral, and extraembryonic mesoderm

abnormal mesendoderm development ( J:80667 )

• at E7.5, homozygotes exhibit limited anterior migration of axial mesendoderm

increased axial mesoderm size ( J:80667 )

• homozygotes display expansion of the axial mesoderm

absent paraxial mesoderm ( J:80667 )

• homozygotes fail to specify paraxial mesoderm

abnormal primitive streak formation ( J:80667 )

• at E7.5, homozygotes display abnormal primitive streak morphology, with nascent mesodermal cells retained within or near a greatly expanded primitive streak