mortality/aging
• frequency of homozygous pups at birth is 2.8%, compared to expected 6.25%; mice do not survive past P0
• number of double homozygotes found at E18.5 is increased over numbers at birth
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embryo
• at E18.5 mutant embryos are smaller than controls and at P0, pups appear runted
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growth/size/body
• at E18.5 mutant embryos are smaller than controls and at P0, pups appear runted
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nervous system
• axon tracts course through cortex aberrantly, oblique to the pial surface
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• definition of marginal zone and cortical subplate has distorted definition
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• cortical plate is misplaced beneath superficial subplate structure
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• complete lack of foliation at E18.5
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• layer is thicker near germinal zone of rhombic lip
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• compact layer of mitral cells is absent at E18.5
• various fiber tracts show disrupted formation; medial lemniscus is intact, but other tracts aren't visible such as the solitary tract
• superior and inferior olivary nuclei are absent
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• indiscernible throughout entire hippocampal formation
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• diffuse laminar organization of CA pyramidal neurons with appearance of cell-free rifts between clusters of cells
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• cortex shows lamination defects and neuronal-positioning abnormalities
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• this layer is absent; Purkinje cells are clustered near posterior lobe of cerebellum at E18.5
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• cerebellum is smaller in size relative to wild-type littermates
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• motor neurons in spinal cord display pathological changes, including chromatolysis at E18.5
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chromatolysis
(
J:71181
)
• seen in some motor neurons at E18.5
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behavior/neurological
• no response to clamp stimulation of tails at P0
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cellular
• axon tracts course through cortex aberrantly, oblique to the pial surface
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