Phenotypes Associated with This Genotype

Genotype
MGI:2654222

• Allelic Composition: Cd46^tm1Osb/Cd46^tm1Osb
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:82516 )

N • homozygous null females were fertile and phenotypically normal

N • homozygous null males displayed normal testes, normal spermatogenesis, and normal fecundity at maturity

increased litter size ( J:82516 )

• surprisingly, the litter size was significantly high when homozygous null males (which had been backcrossed with the C57BL/6 strain for >5 generations) were mated with females of the DBA/2N inbred strain (known for its small litter size)

abnormal fertilization ( J:82516 )

• mutant sperm showed a significantly increased fertilization ratio in superovulated oviducts relative to wild-type

• C3 complement deposition on acrosome-reacted sperm appeared unaffected

• production of double-transgenic mouse lines that carried a homozygous null gene with an additional green sperm transgene showed that mutant sperm exhibit increased competency of spontaneous acrosome reaction