Phenotypes Associated with This Genotype

Genotype
MGI:2451221

• Allelic Composition: Pde4d^tm1Mct/Pde4d^tm1Mct
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Growth comparison of wild type and Pde4d^tm1Mct/Pde4d^tm1Mct mice

mortality/aging

postnatal lethality, incomplete penetrance ( J:58116 )

• 13.8% of homozygous null mice are recovered at D30

growth/size/body

decreased body weight ( J:58116 )

• 30-40% decrease in body weight at 1-2 wks of age relative to wild-type and heterozygous littermates

postnatal growth retardation ( J:58116 )

• growth rate returns to normal at about 2 wks, though the weight of adult mice remains lower than that of wild-type and heterozygous littermates

immune system

immune system phenotype ( J:106828 )

N • response to LPS and thioglycollate is similar to that in wild-type mice

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

impaired neutrophil recruitment ( J:94853 )

• 48% reduction in the number of neutrophils that are recruited to the lung following LPS inhalation compared to wild-type and homozygous Pde4a mutants

abnormal tumor necrosis factor level ( J:94853 )

• levels of TNF-alpha were significantly reduced at 2 hours after LPS inhalation and remained lower for up to 4 hours compared to controls

reproductive system

decreased superovulation rate ( J:58116 )

♀ • impaired ovulation, yielding few oocytes, after administration of gonadotropins; diminished sensitivity of granulosa cells to gonadotropins

reduced female fertility ( J:58116 )

♀

decreased litter size ( J:58116 )

homeostasis/metabolism

abnormal tumor necrosis factor level ( J:94853 )

• levels of TNF-alpha were significantly reduced at 2 hours after LPS inhalation and remained lower for up to 4 hours compared to controls

cellular

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

hematopoietic system

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

impaired neutrophil recruitment ( J:94853 )

• 48% reduction in the number of neutrophils that are recruited to the lung following LPS inhalation compared to wild-type and homozygous Pde4a mutants