Phenotypes Associated with This Genotype

Genotype
MGI:2175791

• Allelic Composition: Crebbp^{Gt(U-San)112Imeg}/Crebbp^{Gt(U-San)112Imeg}
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:54568 )

• embryos obtained from F3 or F4 intercrosses die between E9.5 and E10.5

cardiovascular system

abnormal blood vessel morphology ( J:54568 )

• in the trunk, E9.5 mutants lack sprouting vessels from the dorsal aorta or umbilical vein in parietal mesoderm

• in the head region, E9.5 mutants display a disoriented posterior branch of primary head vein and reduced numbers of blood cells in the dorsal aorta

• ectopic blood cells are detected in the hindgut diverticulum and coelomic cavity

decreased vascular endothelial cell number ( J:54568 )

• in the trunk, E9.5 mutant embryos show reduced endothelial cells in the omphalomesenteric artery and dorsal aorta

abnormal vascular development ( J:53370 , J:54568 )

• organ culture of para-aortic splanchnopleural mesoderm (P-Sp) with stromal cells indicates defects in both vasculogenesis and angiogenesis (J:53370)

• addition of VEGF to mutant cultures partially rescues these defects (J:53370)

failure of vascular branching ( J:54568 )

• at E9.5, homozygotes show gross vascular branching defects in the head and trunk, including the para-aortic splanchnopleural region: both large vessels and smaller vascular branches are absent

decreased angiogenesis ( J:54568 )

abnormal vascular endothelial cell development ( J:53370 )

abnormal vasculogenesis ( J:54568 )

absent organized vascular network ( J:54568 )

• at E9.5, homozygotes lack an organized vascular network

absent vitelline blood vessels ( J:54568 )

• at E9.5, mutant yolk sacs exhibit a significant reduction in vitelline vessels

embryo

embryonic growth arrest ( J:54568 )

• homozygotes become growth arrested at E8.75-E9.0

decreased embryo size ( J:54568 )

• at E9.5, homozygous embryos are smaller than wild-type embryos

open neural tube ( J:54568 )

• at E9.5, homozygous embryos display an open neural tube

abnormal extraembryonic tissue morphology ( J:54568 )

absent vitelline blood vessels ( J:54568 )

• at E9.5, mutant yolk sacs exhibit a significant reduction in vitelline vessels

pale yolk sac ( J:54568 )

• at E9.5, homozygotes display a pale yolk sac

hematopoietic system

anemia ( J:54568 )

• at E9.5, homozygotes exhibit marked anemia and fail to survive beyond the stage of primitive hematopoiesis

impaired hematopoiesis ( J:54568 )

• at E9.5, mutant yolk sacs exhibit 22% and 69%, respectively, of erythroid and granulocyte-macrophage colony-forming capacity relative to wild-type counterparts

• at E9.5, the total number of nucleated erythroid cells present in mutant yolk sacs is reduced to 20% of wild-type

nervous system

open neural tube ( J:54568 )

• at E9.5, homozygous embryos display an open neural tube

growth/size/body

decreased embryo size ( J:54568 )

• at E9.5, homozygous embryos are smaller than wild-type embryos

integument

pallor ( J:54568 )

• at E9.5, homozygous mutant embryos are much paler than wild-type embryos