Analysis Tools
mortality/aging
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• homozygotes are present at the expected Mendelian frequencies from E8.5 to E18.5 but fail to survive beyond P1
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embryo
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• at E11.5, mutant embryos display disrupted somite segment polarity
• failure of sclerotome condensation suggests that the identity of the caudal halves of each segment are not specified
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• at E10-E18, mutant embryos are significantly smaller than wild-type embryos
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• at E9.5, mutant embryos display irregularly-shaped somites along the entire length of the neural tube
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• at E9.5, mutant embryos display absence of somites at the caudal most regions; some somites appear compressed and fused, lacking a symmetric segmentation pattern across the midline
• despite defective somite segmentation, specification of somitic cell lineages (i.e. sclerotome and dermomyotome) appears unaffected at E10.5
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skeleton
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• at E13.5, homozygotes show significant defects in the formation of the vertebral column and ribs
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• at E15.5, homozygotes exhibit abnormal bending of the basioccipital bone; the angle formed between the basioccipital bone and the atlas is distorted
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• at E13.5, homozygotes display defective rib development
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• at E13.5, homozygotes display fusion of the vertebral rudiments
• by E15.5, mutants show a significantly reduced vertebral column with abnormal segmentation adjacent to the spinal cord and fusion of the dorsal arches
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• at E11.5, mutant embryos fail to exhibit sclerotome intrasegmental condensation
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nervous system
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• at E11.5, all homozygotes exhibit hemorrhages beneath the primordial dura and leptomeninges, and in neural parenchyma; rare focal necrosis is observed
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• at E11.5, all homozygotes exhibit hemorrhages within the ventricles; however, overall architecture and cellularity of the brain is preserved
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• at E15.5, mutant DRG appear fused over multiple segments along the craniocaudal axis of the vertebral column
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growth/size/body
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• at E10-E18, mutant embryos are significantly smaller than wild-type embryos
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• at E10-E18, mutant mice are significantly smaller than wild-type mice
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limbs/digits/tail
short tail
(
J:40308
)
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• at E10-E18, mutant embryos exhibit a stubby tail
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cardiovascular system
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• at E11.5, all homozygotes exhibit hemorrhages beneath the primordial dura and leptomeninges, and in neural parenchyma; rare focal necrosis is observed
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• at E11.5, all homozygotes exhibit hemorrhages within the ventricles; however, overall architecture and cellularity of the brain is preserved
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craniofacial
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• at E15.5, homozygotes exhibit abnormal bending of the basioccipital bone; the angle formed between the basioccipital bone and the atlas is distorted
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