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Allele Symbol Allele Name Allele ID |
Tg(H2-Ea-HA)HACIIAjca transgene insertion HACII, Andrew J Caton MGI:5903427 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice develop arthritis more quickly but with disease penetrance and severity similar to double Tg(Tcra/Tcrb)1Vbo/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• mice develop arthritis more quickly but with disease penetrance and severity similar to double Tg(Tcra/Tcrb)1Vbo/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• decrease in the percentages of IL-17- and IFN-gamma-secreting Valpha8.3+VBeta10+ CD4+T cells in the joint-draining lymph nodes of nonarthritic mice
• in mice that develop arthritis despite absence of B cells, the frequencies of IFN-gamma-secreting Valpha8.3+VBeta10+ CD4+T are reduced relative to arthritic double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice, but the frequencies of IL-17-secreting Valpha8.3+VBeta10+CD4+ T cells are not
• nonarthritic mice show lower frequencies of clonotypic, but not of total, IFN-gamma-, and IL-17 producing CD4+ T cells than nonarthritic double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• percentage of CD4+ T cells is increased, however the number of CD4+ T cells expressing the Vslpha8.3+Vbeta10+ clonotypic TCR does not differ from double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• decrease in the overall cellularity of spleens
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• decrease in the percentages of IL-17- and IFN-gamma-secreting Valpha8.3+VBeta10+ CD4+T cells in the joint-draining lymph nodes of nonarthritic mice
• in mice that develop arthritis despite absence of B cells, the frequencies of IFN-gamma-secreting Valpha8.3+VBeta10+ CD4+T are reduced relative to arthritic double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice, but the frequencies of IL-17-secreting Valpha8.3+VBeta10+CD4+ T cells are not
• nonarthritic mice show lower frequencies of clonotypic, but not of total, IFN-gamma-, and IL-17 producing CD4+ T cells than nonarthritic double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• percentage of CD4+ T cells is increased, however the number of CD4+ T cells expressing the Vslpha8.3+Vbeta10+ clonotypic TCR does not differ from double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• decrease in the overall cellularity of spleens
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• decrease in the overall cellularity of the joint-draining lymph nodes
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• mice exhibit decreased arthritis development compared to double Tg(Tcra/Tcrb)#Ajca/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mild inflammatory processes are seen in the hearts of some arthritic mice
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• severe deletion of HA-specific 6.5+CD4+CD8- single-positive thymocytes indicating deletion of autoreactive CD4+ T cells, although a subset of 6.5+CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1 TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• increase in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
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• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)1Vbo/0 mice
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• proinflammatory cytokine levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• interleukin-6 levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• swollen joints show a high degree of synovitis
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• majority of mice spontaneously develop inflammatory arthritis, showing overt joint inflammation and swelling affecting both front and rear paws
• joint inflammation first becomes evident between 6 and 8 weeks of age, and by 14 weeks almost all mice show at least one inflamed paw
• penetrance of arthritis is similar in males and females
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment of prearthritic mice with anti-TNF antibody results in a reduction in arthritis penetrance associated with a reduced accumulation of Th17 cells in the joints but has no effect on CD4+Foxp3+ Tregs
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• mild inflammatory processes are seen in the kidneys of some arthritic mice
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• severe deletion of HA-specific 6.5+CD4+CD8- single-positive thymocytes indicating deletion of autoreactive CD4+ T cells, although a subset of 6.5+CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1 TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• increase in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
|
|
• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)1Vbo/0 mice
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• proinflammatory cytokine levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• interleukin-6 levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• mild inflammatory processes are seen in the hearts of some arthritic mice
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• mild inflammatory processes are seen in the kidneys of some arthritic mice
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• extensive perivascular infiltrates in the lungs of arthritic mice
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• swollen joints show a high degree of synovitis
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• majority of mice spontaneously develop inflammatory arthritis, showing overt joint inflammation and swelling affecting both front and rear paws
• joint inflammation first becomes evident between 6 and 8 weeks of age, and by 14 weeks almost all mice show at least one inflamed paw
• penetrance of arthritis is similar in males and females
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment of prearthritic mice with anti-TNF antibody results in a reduction in arthritis penetrance associated with a reduced accumulation of Th17 cells in the joints but has no effect on CD4+Foxp3+ Tregs
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• swollen joints show a high degree of articular degeneration
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• deletion of HA-specific Valpha8.3+CD4+CD8- thymocytes indicates deletion of autoreactive CD4+ T cells, although a subset of clonotypic CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1(SW) TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• however, no differences between males and females is seen in the percentages of CD4+ T cells, of CD4+CD25+Foxp3+ cells, of CD4+IL-17+ cells, or of B cells in the joint-draining lymph nodesincrease in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells exhibit reduced spleen and joint-draining lymph node cellularities and increased percentages of CD4+ T cells
• treatment with an anti-CD20 mAb results in lower frequencies of clonotypic cytokine-producing T cells in mice that do not develop arthritis
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• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)#/0 mice
• serum IgG titers are highest in arthritic females and are lower in both arthritic and nonarthritic males
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells show reduced levels of serum IgG
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• CD4+ T cells undergo little or no proliferation response to APCs from these mice
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• proinflammatory cytokine levels are decreased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
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• interleukin-17 levels are higher in the serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
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• interleukin-6 levels are decreased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
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| N |
• perivascular infiltrates are not seen in the lungs of arthritic mice and no inflammation in the hearts and kidneys
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|
• deletion of HA-specific Valpha8.3+CD4+CD8- thymocytes indicates deletion of autoreactive CD4+ T cells, although a subset of clonotypic CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1(SW) TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• however, no differences between males and females is seen in the percentages of CD4+ T cells, of CD4+CD25+Foxp3+ cells, of CD4+IL-17+ cells, or of B cells in the joint-draining lymph nodesincrease in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells exhibit reduced spleen and joint-draining lymph node cellularities and increased percentages of CD4+ T cells
• treatment with an anti-CD20 mAb results in lower frequencies of clonotypic cytokine-producing T cells in mice that do not develop arthritis
|
|
• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)#/0 mice
• serum IgG titers are highest in arthritic females and are lower in both arthritic and nonarthritic males
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells show reduced levels of serum IgG
|
|
• CD4+ T cells undergo little or no proliferation response to APCs from these mice
|
|
• proinflammatory cytokine levels are decreased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
|
|
• interleukin-17 levels are higher in the serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
|
|
• interleukin-6 levels are decreased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)#Ajca/0 mice
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• joint-draining lymph nodes of arthritic females are larger than either arthritic or nonarthritic males
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• swollen joints show a high degree of synovitis
• males show lower synovitis and articular degeneration than females
|
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• adult mice develop overt joint swelling affecting both front and hind paws
• males exhibit a lower penetrance of arthritis, a delay in disease onset, and decreased severity of arthritis relative to females
• mice that do not show overt joint swelling do not exhibit synovitis or articular degeneration
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment with an anti-IL-17R mAb abrogates arthritis development in females
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells prevents arthritis development in the majority of mice
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|
• swollen joints show a high degree of synovitis
• males show lower synovitis and articular degeneration than females
|
|
• adult mice develop overt joint swelling affecting both front and hind paws
• males exhibit a lower penetrance of arthritis, a delay in disease onset, and decreased severity of arthritis relative to females
• mice that do not show overt joint swelling do not exhibit synovitis or articular degeneration
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment with an anti-IL-17R mAb abrogates arthritis development in females
• 5-6 week old mice treated with an anti-CD20 mAb to eliminate B cells prevents arthritis development in the majority of mice
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• swollen joints show a high degree of articular degeneration
• males show lower synovitis and articular degeneration than females
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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