Phenotypes associated with this allele
Allelic
Composition |
Ntstm1(cre)Mgmj/Nts+
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Genetic
Background |
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ntstm1(cre)Mgmj mutation
(1 available);
any
Nts mutation
(18 available)
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growth/size/body
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• mice show slightly decreased body weight relative to controls
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adipose tissue
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• mice show slightly decreased adiposity relative to controls
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nervous system
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• all lateral hypothalamic area (LHA) Nts +ve neurons treated with leptin become hyperpolarized; in controls, about 20% of wild-type LHA Nts +ve neurons treated with leptin while about 60% depolarize upon leptin treatment
• this indicates that deletion of Lepr from Lepr, Nts +ve neurons abrogates the direct depolarization response
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growth/size/body
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• males have decreased lean mass as a proportion of body mass compared to controls at 12-15 weeks
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• treatment with leptin for 24 hours reduces weight of control animals, but action of leptin is blunted in mutants
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• at 12-15 weeks, mice show increased body weight relative to controls
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adipose tissue
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• mice show higher adiposity at 12-15 weeks
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• gonadal fat pad white adipose tissue (WAT) weight is increased relative to controls at 12-15 weeks
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• mice have increased % fat mass/total body mass compared to controls at 12-15 weeks
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behavior/neurological
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• in response to injection of amphetamine (AMPH), locomotor activity rapidly increases in controls, but effect is blunted in Lepr mutants
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• mice exhibit decreased ambulatory activity across the light-dark cycle compared to controls
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nervous system
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• fasting does not increase activity of orexin neurons (measured by c-fos expression) in mutants in contrast to induction of robust activity in control animals
• treatment with leptin for 24 hours increases orexin expression by neurons in controls but not in mutants
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• amphetamine-evoked dopamine release in the nucleus accumbens, NAc, is reduced (reduced amplitude of dopamine peak and increased dopamine biological half-life measured by amperometry) suggesting dopamine transporter activity is decreased in the NAc, contributing to reduceAMPH-induced activity
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homeostasis/metabolism
nervous system
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N |
• 62% of brain slice lateral hypothalamic area (LHA) Nts +ve neurons depolarize when treated with leptin while about 20% of Nts +ve neurons respond with slow hyperpolarization
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Analysis Tools