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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
E4f1tm1.1Llca
targeted mutation 1.1, Laurent Le Cam
MGI:4867860
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Cdkn2atm1Rdp/Cdkn2atm1Rdp
E4f1tm1Pisc/E4f1tm1.1Llca
Polr2atm1(cre/ERT2)Bbd/Polr2a+
involves: 129/Sv * 129S4/SvJae * C57BL/6 * SJL MGI:4867863
cn2
E4f1tm1Pisc/E4f1tm1.1Llca
Tg(KRT5-cre)5132Jlj/0
involves: 129S4/SvJae * C57BL/6 * DBA/2J MGI:4867862
cn3
E4f1tm1Pisc/E4f1tm1.1Llca
Polr2atm1(cre/ERT2)Bbd/Polr2a+
involves: 129S4/SvJae * C57BL/6 * SJL MGI:4867861


Genotype
MGI:4867863
cn1
Allelic
Composition
Cdkn2atm1Rdp/Cdkn2atm1Rdp
E4f1tm1Pisc/E4f1tm1.1Llca
Polr2atm1(cre/ERT2)Bbd/Polr2a+
Genetic
Background
involves: 129/Sv * 129S4/SvJae * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm1Rdp mutation (6 available); any Cdkn2a mutation (57 available)
E4f1tm1.1Llca mutation (2 available); any E4f1 mutation (26 available)
E4f1tm1Pisc mutation (1 available); any E4f1 mutation (26 available)
Polr2atm1(cre/ERT2)Bbd mutation (3 available); any Polr2a mutation (52 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
integument
• development of hyperkeratosis following 4-hydroxytamoxifen treatment is reduced and delayed compared to mutant mice wild-type for Cdkn2a
• development of hyperplasia following 4-hydroxytamoxifen treatment is reduced and delayed compared to mutant mice wild-type for Cdkn2a
• partial restoration in long term outgrowth of 4-hydroxytamoxifen exposed keratinocytes in culture compared to mutant mice wild-type for Cdkn2a




Genotype
MGI:4867862
cn2
Allelic
Composition
E4f1tm1Pisc/E4f1tm1.1Llca
Tg(KRT5-cre)5132Jlj/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E4f1tm1.1Llca mutation (2 available); any E4f1 mutation (26 available)
E4f1tm1Pisc mutation (1 available); any E4f1 mutation (26 available)
Tg(KRT5-cre)5132Jlj mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

homeostasis/metabolism
• die exhibiting signs of acute dehydration

integument
• in skin grafted onto nude mice
• epidermal hypocellularity develops in skin grafted onto nude mice
• fewer epidermal stem cells are present
• develops in skin grafted onto nude mice
• develops by P3 - P4
• no abnormal cell death is seen in these lesions
• hyperproliferation and increased mitotic index in epidermal basal cells
• after 10 - 15 days in culture keratinocytes fail to develop typical holoclones (corresponding to long term clonal outgrowth of epidermal stem cells)




Genotype
MGI:4867861
cn3
Allelic
Composition
E4f1tm1Pisc/E4f1tm1.1Llca
Polr2atm1(cre/ERT2)Bbd/Polr2a+
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
E4f1tm1.1Llca mutation (2 available); any E4f1 mutation (26 available)
E4f1tm1Pisc mutation (1 available); any E4f1 mutation (26 available)
Polr2atm1(cre/ERT2)Bbd mutation (3 available); any Polr2a mutation (52 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• after topical application of 4-hydroxytamoxifen

integument
• topical application of 4-hydroxytamoxifen to the unshaved tail results in complete alopecia by 6 weeks after application
• fewer hair follicle bulges are detected 4 - 6 weeks after topical application of 4-hydroxytamoxifen
• at 2 - 3 weeks after topical application of 4-hydroxytamoxifen the long term retaining cell region is expanded but by 6 weeks after application long term retaining cells appear to be lost
• broad disorganization of the interfollicular epithelium develops by 3 - 4 weeks after topical application of 4-hydroxytamoxifen
• hyperkeratosis is associated with loss of cellularity in the interfollicular epithelium
• develops by 3 - 4 weeks after topical application of 4-hydroxytamoxifen
• after topical application of 4-hydroxytamoxifen
• after topical application of 4-hydroxytamoxifen
• massive hyperplasia with increased cellularity in the interfollicular epithelium and the infundibulum in the first few weeks after topical application of 4-hydroxytamoxifen
• no abnormal cell death is seen in these lesions
• by 2 - 4 weeks after topical application of 4-hydroxytamoxifen, severe skin ulcerative lesions develop
• between 1 - 2 weeks after topical application of 4-hydroxytamoxifen, back skin is ruffled and wrinkled
• between 1 - 2 weeks after topical application of 4-hydroxytamoxifen, back skin is thickened
• increase in the proportion of proliferating epidermal cells in the first few weeks after topical application of 4-hydroxytamoxifen
• after 10 - 15 days in culture keratinocytes from 4-hydroxytamoxifen treated skin fail to develop typical holoclones (corresponding to long term clonal outgrowth of epidermal stem cells)





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory