Analysis Tools
skeleton
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IMPC - WTSI
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IMPC - WTSI
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Allele Symbol Allele Name Allele ID |
Brdttm1a(EUCOMM)Wtsi targeted mutation 1a, Wellcome Trust Sanger Institute MGI:4455954 |
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| Summary |
3 genotypes
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Data Sources
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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IMPC - WTSI
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IMPC - WTSI
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls
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• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
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• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
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• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls
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• disruption of spermatogenesis before the first meiotic division, consistent with presence of pachytene spermatocytes at 8 weeks of age, as revealed by gamma-H2AX labeling
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• complete absence of epididymal sperm
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• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
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• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
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• meiotic arrest at the spermatocyte stage at 8 weeks of age
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• male homozygotes display delayed puberty, as shown by normal testosterone levels and accessory gland weights at 14 and 28 weeks of age
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• continuous mating of male homozygotes with fertile females does not result in pregnancy
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• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
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• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
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• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls
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• significantly decreased plasma testosterone levels at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
• however, no changes in Leydig cell number and/or size are detected between 8 and 28 weeks of age
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• complete absence of epididymal sperm
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• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
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• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
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• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• testes lack post-meiotic cells
• meiosis I is not completed
• however, late pachytene stage occurs normally
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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