About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd226tm1.1Cln
targeted mutation 1.1, Marco Colonna
MGI:4454325
Summary 2 genotypes


Genotype
MGI:4454326
hm1
Allelic
Composition		 Cd226tm1.1Cln/Cd226tm1.1Cln
Genetic
Background		 B6.129P2-Cd226tm1.1Cln
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd226tm1.1Cln mutation (0 available); any Cd226 mutation (35 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr

hematopoietic system
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr
impaired natural killer cell mediated cytotoxicity ( J:159749 )
• NK cells do not appreciably kill B16F10 tumor cells
• however, the ability to kill YAC-1 cells is unimpaired

immune system
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr
impaired natural killer cell mediated cytotoxicity ( J:159749 )
• NK cells do not appreciably kill B16F10 tumor cells
• however, the ability to kill YAC-1 cells is unimpaired
decreased inflammatory response ( J:209990 )
• significantly increased survival rates after LPS-induced endotoxicosis at 0.5 or 0.75 mg LPS/30 g levels

neoplasm
increased metastatic potential ( J:159749 )
• with lower tumor doses mice have significantly higher numbers of B16F10 lung metastases than do wild-type
• the injection of the mouse prostate carcinoma, RM-1, at all tumor doses inoculated, results in significantly higher numbers of RM-1 lung metastases than in wild-type
increased tumor growth/size ( J:159749 )
• accelerated tumor growth is observed after subcutaneous injection of B16F10 compared to wild-type
• unlike wild-type mice, mice fail to reject the subcutaneous injection of a colon carcinoma cell line, MC38,that has been transfected with OVA
abnormal tumor susceptibility ( J:209990 )
• more lung metastases after injection of B16F10 melanoma cells into tail vein




Genotype
MGI:4454328
cx2
Allelic
Composition		 Cd226tm1.1Cln/Cd226tm1.1Cln
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 B6.Cg-Cd226tm1.1Cln Tg(TcraTcrb)1100Mjb
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd226tm1.1Cln mutation (0 available); any Cd226 mutation (35 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased cytotoxic T cell cytolysis ( J:159749 )
• CD8 T cells kill B16 cells pulsed with OVA peptide 257-264 less effectively than control CD8 T cells
abnormal T cell activation ( J:159749 )
• CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with Pvr and pulsed with OVA peptide 257-264 fail to become activated
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type

immune system
decreased cytotoxic T cell cytolysis ( J:159749 )
• CD8 T cells kill B16 cells pulsed with OVA peptide 257-264 less effectively than control CD8 T cells
abnormal T cell activation ( J:159749 )
• CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with Pvr and pulsed with OVA peptide 257-264 fail to become activated
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type
decreased interferon-gamma secretion ( J:159749 )
• fewer IFN gamma secreting T cells are evident in lymph nodes after restimulation with the T cell lymphoma cell line EL-4 cells transfected with Pvr and pulsed with OVA peptide 257-264
• however, the frequency of IFN gamma secreting cells is similar to wild-type after restimulation with dendritic cells

cellular
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory