Phenotypes associated with this allele
Allelic
Composition |
Bhlhe22tm1Meg/Bhlhe22tm1Meg
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Genetic
Background |
either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6) or (involves: 129/Sv * CD-1) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
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behavior/neurological
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• excessive licking along with scratching results in skin lesions in most animals
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• resulting in skin lesions in most animals
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• mice show significantly greated scratching behavior than wild-type in response to all pruritic agents
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• in response to intraplantar formalin injection, the initial early phase reaction is similar to controls, but the late phase response is significantly enhanced with the duration of licking behavior is 4 fold greater in mutants; intradermal injection in the cheek induces a scratching (itching sensation) response rather than a wiping response (pain sensation)
• mice show similar responses to nociceptive sensory assays (mechanical sensitivity, thermal sensitivity, inflammation-induced mechanical hypersensitivity) as wild-type animals at 4 weeks of age
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nervous system
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• elevated neural activity associated with the presence of skin lesions is observed in the dorsal horn of the spinal cord; c-Fos activity is upregulated
• no neuropathy or abnormal innervation of the skin is observed
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integument
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• by 4 weeks of age, one third of animals display self-inflicted skin lesions frequently observed on the perineum and haunches; at 8 weeks, almost all animals have lesions
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
|
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nervous system
|
N |
• mice exhibit normal cortical layering
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• axons from corticospinal motor neurons terminal prematurely and fail to enter the spinal cord unlike in wild-type mice
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• mice exhibit a reduction of the corticospinal tract compared with wild-type mice
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behavior/neurological
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• resulting in skin lesions at 6 weeks of age
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• a few mice exhibit a handstand phenotype (walking on forepaws) secondary to hindpaw contraction
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integument
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• due to excessive scratching at 6 weeks of age
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cellular
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• axons from corticospinal motor neurons terminal prematurely and fail to enter the spinal cord unlike in wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Bhlhe22tm3.1(cre)Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation
(2 available)
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nervous system
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• in the superficial laminae of spinal cord, there are significantly fewer lacZ-marked neurons compared to controls; significant loss of cell bodies and neuropil is observed
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Bhlhe22tm3.1(cre)Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Gt(ROSA)26Sortm1Sor mutation
(8 available);
any
Gt(ROSA)26Sor mutation
(942 available)
|
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nervous system
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• in the superficial dorsal horn, there are significantly fewer ( about 50%) lacZ-marked neurons compared to controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Bhlhe22tm3.1(cre)Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Gt(ROSA)26Sortm1(EYFP)Cos mutation
(11 available);
any
Gt(ROSA)26Sor mutation
(942 available)
|
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nervous system
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• quantification of neurons at P0 shows a 50% decrease in number of marked neurons in superficial dorsal horn compared to controls with no difference in numbers in any other spinal cord region
• significant increase in number of apoptotic cells (marked neurons) in superficial dorsal horn is observed at E18.5, but no difference is observed at E17.5 or 19.5
• partial loss of glutamatergic and GABAergic neurons is observed in dorsal horn
• number of marked neurons in dorsal horn are not different from controls when BrdU labeling is done at E12.5 or E13.5 and analysis is done at E17.5, indicating that neuronal mitosis is not affected
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bhlhe22tm1Meg mutation
(0 available);
any
Bhlhe22 mutation
(12 available)
Cdh11tm1Mta mutation
(1 available);
any
Cdh11 mutation
(42 available)
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integument
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• at 13 weeks, delayed compared to in Bhlhe22tm1Meg homozygotes
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nervous system
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• mice exhibit a reduction of the corticospinal tract compared with wild-type mice although not as severe as in Bhlhe22tm1Meg homozygotes
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Analysis Tools