Analysis Tools
cellular
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• after adenoviral cre infection, MEFs are devoid of fibronectin fibrils
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Allele Symbol Allele Name Allele ID |
Slc3a2tm1.1Mgin targeted mutation 1, Mark H Ginsberg MGI:3843272 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• after adenoviral cre infection, MEFs are devoid of fibronectin fibrils
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• despite Cre mediated deletion in B cells, mice have normal pre-B, pro-B, immature and mature B cell numbers and normal splenic architecture
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• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells
• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal
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• decrease in germinal center B cell and plasma cell numbers 1 week after immunization
• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers
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• impaired antibody secretion following LPS stimulation
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• impaired class switching following LPS stimulation
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• activated B cells fail to spread after antibody mediated ligation of integrins
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• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells
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• decrease in basal and antigen specific levels of IgG
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• decrease in antigen specific levels of IgM
• however, basal IgM levels are similar to controls
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• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells
• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal
|
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• decrease in germinal center B cell and plasma cell numbers 1 week after immunization
• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers
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• impaired antibody secretion following LPS stimulation
|
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• impaired class switching following LPS stimulation
|
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• activated B cells fail to spread after antibody mediated ligation of integrins
|
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• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells
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• decrease in basal and antigen specific levels of IgG
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• decrease in antigen specific levels of IgM
• however, basal IgM levels are similar to controls
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• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• decrease in antigen specific levels of IgG3
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• decrease in antigen specific levels of IgM
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• decrease in antigen specific levels of IgG3
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• decrease in antigen specific levels of IgM
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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