About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Slc3a2tm1.1Mgin
targeted mutation 1, Mark H Ginsberg
MGI:3843272
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Slc3a2tm1.1Mgin/Slc3a2tm1.1Mgin involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3843273
cn2
Cd19tm1(cre)Cgn/Cd19+
Slc3a2tm1.1Mgin/Slc3a2tm1.1Mgin
involves: 129 * C57BL/6 * SJL MGI:3843276
cn3
Cd19tm1(cre)Cgn/Cd19+
Slc3a2tm1.1Mgin/Slc3a2tm1Yai
involves: 129 * C57BL/6 * SJL MGI:3843277


Genotype
MGI:3843273
cn1
Allelic
Composition
Slc3a2tm1.1Mgin/Slc3a2tm1.1Mgin
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc3a2tm1.1Mgin mutation (0 available); any Slc3a2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• after adenoviral cre infection, MEFs are devoid of fibronectin fibrils




Genotype
MGI:3843276
cn2
Allelic
Composition
Cd19tm1(cre)Cgn/Cd19+
Slc3a2tm1.1Mgin/Slc3a2tm1.1Mgin
Genetic
Background
involves: 129 * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (8 available); any Cd19 mutation (15 available)
Slc3a2tm1.1Mgin mutation (0 available); any Slc3a2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells

immune system
N
• despite Cre mediated deletion in B cells, mice have normal pre-B, pro-B, immature and mature B cell numbers and normal splenic architecture
• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal
• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells
• decrease in germinal center B cell and plasma cell numbers 1 week after immunization
• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers
• impaired antibody secretion following LPS stimulation
• impaired class switching following LPS stimulation
• activated B cells fail to spread after antibody mediated ligation of integrins
• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells
• decrease in basal and antigen specific levels of IgG
• decrease in antigen specific levels of IgM
• however, basal IgM levels are similar to controls

hematopoietic system
• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells
• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal
• decrease in germinal center B cell and plasma cell numbers 1 week after immunization
• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers
• impaired antibody secretion following LPS stimulation
• impaired class switching following LPS stimulation
• activated B cells fail to spread after antibody mediated ligation of integrins
• minimal proliferation in response to anti-IgM antibody stimulation
• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells
• decrease in basal and antigen specific levels of IgG
• decrease in antigen specific levels of IgM
• however, basal IgM levels are similar to controls




Genotype
MGI:3843277
cn3
Allelic
Composition
Cd19tm1(cre)Cgn/Cd19+
Slc3a2tm1.1Mgin/Slc3a2tm1Yai
Genetic
Background
involves: 129 * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (8 available); any Cd19 mutation (15 available)
Slc3a2tm1.1Mgin mutation (0 available); any Slc3a2 mutation (13 available)
Slc3a2tm1Yai mutation (0 available); any Slc3a2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• decrease in antigen specific levels of IgG3
• decrease in antigen specific levels of IgM

hematopoietic system
• decrease in antigen specific levels of IgG3
• decrease in antigen specific levels of IgM





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
03/24/2020
MGI 6.15
The Jackson Laboratory