Analysis Tools
cellular
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• T cells infected with a retroviral cre exhibit cell death without inducing cell death in uninfected cells
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Allele Symbol Allele Name Allele ID |
Gt(ROSA)26Sortm1(DTA)Lky targeted mutation 1, Richard M Locksley MGI:3829362 |
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| Summary |
8 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• T cells infected with a retroviral cre exhibit cell death without inducing cell death in uninfected cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in the lungs of mice infected with N. brasiliensis
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• mice exhibit reduced helminthes Nippostrongylus brasiliensis expulsion compared with control mice
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• in the lungs of mice infected with N. brasiliensis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in the lungs of mice infected with N. brasiliensis
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• slightly less in an anti-NP response
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• in lung CD4+ T cells of mice infected with N. brasiliensis
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• in lung CD4+ T cells of mice infected with N. brasiliensis
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• mice fail to expel the helminth Nippostrongylus brasiliensis
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• in the lungs of mice infected with N. brasiliensis
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• slightly less in an anti-NP response
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• die around E15.5
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• palatal shelves are smaller
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• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level
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• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles
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• absence of levator veli palatini muscle
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• absence of palatopharyngeus muscle
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• absence of tensor veli palatini muscle
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• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles
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• absence of levator veli palatini muscle
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• absence of palatopharyngeus muscle
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• absence of tensor veli palatini muscle
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• absence of superior pharyngeal constrictor muscle
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• palatal shelves are smaller
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• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level
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• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles
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• absence of levator veli palatini muscle
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• absence of palatopharyngeus muscle
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• absence of tensor veli palatini muscle
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• palatal shelves are smaller
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• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level
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• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles
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• absence of levator veli palatini muscle
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• absence of palatopharyngeus muscle
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• absence of tensor veli palatini muscle
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• absence of superior pharyngeal constrictor muscle
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no increase in type 2 innate lymphoid cells (ILC2) or induction of eotaxin-1 (Ccl11) is detected in lungs in response to administration of Il2 and Il33 in contrast to control mice lacking DTA expression which have a higher ILC2 population and Ccl11 expression
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• viable double mutant embryos are recovered in significantly reduced numbers at E14.5 and E17.5 compared to cre-negative/R26DTA embryos
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• the few animals surviving past birth are smaller than littermates
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| N |
• ACTH hormone levels are not significantly altered in mutants at E14.5
• at E17.5 and P0, numbers of ACTH, TPIT, PIT-1 (ie. thyrotropes, somatotropes, and lactotropes), PRL, and GH-positive cells in the anterior pituitary are not significantly reduced compared to controls
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• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary
• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip
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• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary
• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also
• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased
• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls
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• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary
• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip
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• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary
• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also
• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased
• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls
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• numbers of Lhx-positive interneurons are decreased in the V2 interneuron region of the spinal cord at E14.5, but populations of motorneurons (ventral) are not affected
• at E14.5, V2a interneurons expressing LHX3 and CHX10 are reduced in number
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• of 2 surviving male mutants, one was infertile
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• peripheral blood is deficient in eosinophils relative to wild-type (0.19% vs 1.85%)
• other non-eosinophilic circulating cell populations are unchanged in cell number and relative composition in mutants
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• peripheral blood is deficient in eosinophils relative to wild-type (0.19% vs 1.85%)
• other non-eosinophilic circulating cell populations are unchanged in cell number and relative composition in mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• despite the reduction in CD4+ and CD8+ T cells, the pool of memory T cells is normal
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• CD8+ cells exhibit an immature phenotype due to incomplete thymic maturation
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• T cells exhibit incomplete repertoires of TCR specificities with a biased Vbeta chain usage unlike in wild-type cells
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• the naive T cell population in the peripheral pool is depleted and mice remain lymphopenic over time
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• by 50%
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• CD4+ cells in the thymus are almost completely absent
• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination
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• fewer immature CD8+ T cells are found in the thymus compared to in wild-type mice
• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination
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• mice exhibit a loss of CD4+ NK T cells in the liver compared to in wild-type mice
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• the spleen has a smaller T cell zone than in wild-type mice
• however, overall architecture is normal
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• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells
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• the lymph nodes have smaller T cell zones than in wild-type mice
• however, overall architecture is normal
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• mice infected with N. brasiliensis exhibit impaired effector cell recruitment and worm expulsion with 4-fold higher worm counts in the small intestine compared to in similarly treated wild-type mice
• nine days after infection with N. brasiliensis, mice exhibit reduced basophil and eosinophil frequencies in the blood and lungs compared to similarly treated wild-type mice
• after infection with N. brasiliensis, total numbers of eosinophils, basophils and CD4+ T cells in the lungs are 5- to 10-fold lower than in similarly treated wild-type mice
• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells
• however, IgE levels in mice infected with N. brasiliensis are normal
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• CD8+ cells exhibit an immature phenotype due to incomplete thymic maturation
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• T cells exhibit incomplete repertoires of TCR specificities with a biased Vbeta chain usage unlike in wild-type cells
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• the naive T cell population in the peripheral pool is depleted and mice remain lymphopenic over time
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• by 50%
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• CD4+ cells in the thymus are almost completely absent
• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination
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• fewer immature CD8+ T cells are found in the thymus compared to in wild-type mice
• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination
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• mice exhibit a loss of CD4+ NK T cells in the liver compared to in wild-type mice
|
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• the spleen has a smaller T cell zone than in wild-type mice
• however, overall architecture is normal
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• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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