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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Pomc-MAPT/Topaz)1Rck
transgene insertion 1, Jeffrey M Friedman
MGI:3785478
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Lepob/Lepob
Tg(Pomc-MAPT/Topaz)1Rck/0
involves: C57BL/6 * CBA MGI:3785527
cx2
Tg(Pomc-MAPT/Topaz)1Rck/0
Ucp2Gt(S20-3G1)Sor/Ucp2Gt(S20-3G1)Sor
involves: C57BL/6 * CBA MGI:3811501
tg3
Tg(Pomc-MAPT/Topaz)1Rck/0 involves: C57BL/6 * CBA MGI:3785524


Genotype
MGI:3785527
cx1
Allelic
Composition
Lepob/Lepob
Tg(Pomc-MAPT/Topaz)1Rck/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lepob mutation (5 available); any Lep mutation (17 available)
Tg(Pomc-MAPT/Topaz)1Rck mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• treatment with 25 ug/g of leptin results in significant decrease in body weight in Lepob homozygotes compared to wild-type littermates

nervous system
• Lepob homozygotes have a lower total number of synapses onto perikarya of Arcuate nucleus Pomc neurons compared to wild-type littermates; in wild-type, exciitory synapses on Pomc neurons are more numerous than inhibitory synapses, whereas in Lepob homozygotes, inhibitory synapses on Pomc neurons are more numerous
• altered synaptic profile is consistent with increased inhibitory tone on Pomc neurons in Lepob homozygotes
• 6 hours after leptin injection, an increase in total number of synapses on Pomc perikarya is detected, with an increase in excitatory inputs to Pomc cells; after 2 days of leptin treatment, synapse number onto Pomc neurons is doubled with a 300% increase in excitatory synapse number in Lepob homozygotes
• similar changes in synaptic profile are observed after 12 days of treatment
• after 4 days of twice-daily injections of 10 ug of ghrelin, number of excitatory inputs on Pomc neurons is decreased and number of inhibitory inputs is increased compared to saline-treated Lep mice (opposite to effect of leptin treatment)
• in Lepob homozygotes, there are 2-fold more inhibitory postsynaptic currents (IPSCs) as excitatory postsynaptic currents (EPSCs) onto Pomc neurons in the Arcuate nucleus in hypothalamic slices compared to wild-type mice
• 2 days of leptin treatment decreased frequency of spontaneous IPSCs onto Pomc neurons in Lepob homozygotes

behavior/neurological
• treatment with 25 ug/g of leptin results in significant decrease in food intake after 2 and 12 days treatment
• after 4 days of twice-daily injections of 10 ug of ghrelin, an increase in food intake is observed




Genotype
MGI:3811501
cx2
Allelic
Composition
Tg(Pomc-MAPT/Topaz)1Rck/0
Ucp2Gt(S20-3G1)Sor/Ucp2Gt(S20-3G1)Sor
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pomc-MAPT/Topaz)1Rck mutation (1 available)
Ucp2Gt(S20-3G1)Sor mutation (0 available); any Ucp2 mutation (36 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• ghrelin induced recruitment of miniature inhibitory postsynaptic currents on GFP-labeled cells within the hypothalamus was significantly elevated in wild-type animals but not in these mice
• grehlin increases the number of symmetric (inhibitory) synapses and decreases the number of asymmetric (excitatory) synapases found in hypothalamus in wild-type but not mutant mice




Genotype
MGI:3785524
tg3
Allelic
Composition
Tg(Pomc-MAPT/Topaz)1Rck/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pomc-MAPT/Topaz)1Rck mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice display no obvious phenotypic abnormalities





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory