All Phenotypes Tox<tm1.1Kay> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tox^tm1.1Kay/Tox^tm1.1Kay	involves: 129S6/SvEvTac	MGI:3807778
hm2	Tox^tm1.1Kay/Tox^tm1.1Kay	involves: 129S6/SvEvTac * C57BL/6	MGI:4839073
cx3	B2m^tm1Jae/B2m^tm1Jae Tox^tm1.1Kay/Tox^tm1.1Kay	involves: 129S6/SvEvTac * 129S2/SvPas	MGI:3807779
cx4	Tg(Tox)1Kay/? Tox^tm1.1Kay/Tox^tm1.1Kay	involves: 129S6/SvEvTac * C57BL/6	MGI:3807781
cx5	Tg(DO11.10)10Dlo/? Tox^tm1.1Kay/Tox^tm1.1Kay	involves: 129S/Sv * BALB/c * C3H * C57BL/6	MGI:3807780

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD4-positive T cell differentiation ( J:131287 )

• CD4 T cell development is blocked at the double positive stage 3 step where high surface expression of TCR beta is needed to develop precursors into mature CD4⁺ T cells

• analysis of various developmental markers (Bim, Rag1, Gata3, Zbtb7b) places developmental arrest of CD4 T cells after positive selection of the TCR but before CD4 T cell lineage commitment

• this defect is intrinsic to the T cell precursors as revealed by mixed bone marrow chimera experiments in irradiated wild-type hosts

decreased CD4-positive, alpha-beta T cell number ( J:131287 )

• CD4⁺ and CD4+CD8^lo thymocytes are virtually absent in the thymus

• there are 24- and 16-fold reductions in the frequency and absolute number of splenic CD4⁺ T cells compared to wild-type controls

• splenic CD4⁺ T cells are also CD44⁺ indicating a large expansion of a few CD4 T cells that manage to develop in the thymus

decreased NK T cell number ( J:131287 )

• NKT cells are severely reduced both in the thymus and spleen

abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology ( J:131287 )

• the frequency of Foxp3⁺ T cells in the thymus is reduced more than 10-fold

• few Foxp3⁺ T cells are found in the spleen with the many of these cells not expression CD4 or CD25

abnormal CD8-positive, alpha beta T cell morphology ( J:131287 )

• 42% of CD8⁺ T cells are also CD44⁺ indicating an expansion of the CD8⁺ T cell population that leaves the thymus

decreased cytotoxic T cell cytolysis ( J:131287 )

• CD8⁺ T cells have a some reduction in cytoxic activity compared to controls

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:131287 )

• CD4 T cell development is blocked at the double positive stage 3 step where high surface expression of TCR beta is needed to develop precursors into mature CD4⁺ T cells

• analysis of various developmental markers (Bim, Rag1, Gata3, Zbtb7b) places developmental arrest of CD4 T cells after positive selection of the TCR but before CD4 T cell lineage commitment

• this defect is intrinsic to the T cell precursors as revealed by mixed bone marrow chimera experiments in irradiated wild-type hosts

decreased CD4-positive, alpha-beta T cell number ( J:131287 )

• CD4⁺ and CD4+CD8^lo thymocytes are virtually absent in the thymus

• there are 24- and 16-fold reductions in the frequency and absolute number of splenic CD4⁺ T cells compared to wild-type controls

• splenic CD4⁺ T cells are also CD44⁺ indicating a large expansion of a few CD4 T cells that manage to develop in the thymus

decreased NK T cell number ( J:131287 )

• NKT cells are severely reduced both in the thymus and spleen

abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology ( J:131287 )

• the frequency of Foxp3⁺ T cells in the thymus is reduced more than 10-fold

• few Foxp3⁺ T cells are found in the spleen with the many of these cells not expression CD4 or CD25

abnormal CD8-positive, alpha beta T cell morphology ( J:131287 )

• 42% of CD8⁺ T cells are also CD44⁺ indicating an expansion of the CD8⁺ T cell population that leaves the thymus

decreased cytotoxic T cell cytolysis ( J:131287 )

• CD8⁺ T cells have a some reduction in cytoxic activity compared to controls

Allelic Composition	Tox^tm1.1Kay/Tox^tm1.1Kay
Genetic Background	involves: 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tox^tm1.1Kay mutation (0 available); any Tox mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:164687 )

• a T cell-intrinsic block in thymic positive selection is evident in wild-type mice reconstituted with mutant bone marrow

decreased NK cell number ( J:164687 )

• in bone marrow and spleen

abnormal spleen morphology ( J:164687 )

decreased Peyer's patch number ( J:164687 )

small Peyer's patches ( J:164687 )

absent mesenteric lymph nodes ( J:164687 )

absent axillary lymph nodes ( J:164687 )

absent inguinal lymph nodes ( J:164687 )

absent popliteal lymph nodes ( J:164687 )

hematopoietic system

abnormal positive T cell selection ( J:164687 )

• a T cell-intrinsic block in thymic positive selection is evident in wild-type mice reconstituted with mutant bone marrow

decreased NK cell number ( J:164687 )

• in bone marrow and spleen

abnormal spleen morphology ( J:164687 )

digestive/alimentary system

abnormal small intestine morphology ( J:164687 )

• Absence of B220-, CD45+, CD3-, CD4+, CD127- LTi cells in neonatal spleen and small intestine of E18 mice

Allelic Composition	B2m^tm1Jae/B2m^tm1Jae Tox^tm1.1Kay/Tox^tm1.1Kay
Genetic Background	involves: 129S6/SvEvTac * 129S2/SvPas

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2m^tm1Jae mutation (8 available); any B2m mutation (122 available)
Tox^tm1.1Kay mutation (0 available); any Tox mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell number ( J:131287 )

• about twice as much CD8⁺ T cells are found in the thymus of double knockouts compared to mice homozygote for just the B2m^tm1Jae allele

• about 5-fold more CD8⁺ T cells are found in the spleen of double knockouts compared to mice homozygote for just the B2m^tm1Jae allele

• numbers of CD8⁺ T cells in both the thymus and spleen are still well below normal numbers indicating the vast majority of CD8⁺ T cells are Class I-dependent

hematopoietic system

abnormal CD8-positive, alpha-beta T cell number ( J:131287 )

• about twice as much CD8⁺ T cells are found in the thymus of double knockouts compared to mice homozygote for just the B2m^tm1Jae allele

• about 5-fold more CD8⁺ T cells are found in the spleen of double knockouts compared to mice homozygote for just the B2m^tm1Jae allele

• numbers of CD8⁺ T cells in both the thymus and spleen are still well below normal numbers indicating the vast majority of CD8⁺ T cells are Class I-dependent

Allelic Composition	Tg(Tox)1Kay/? Tox^tm1.1Kay/Tox^tm1.1Kay
Genetic Background	involves: 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Tox)1Kay mutation (0 available)
Tox^tm1.1Kay mutation (0 available); any Tox mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell number ( J:131287 )

• transgene expression rescues the reduced number of CD4⁺ T cells found in the Tox^tm1.1Kay homozygotes

• the percentage of thymic single positive CD8⁺ T cells are enhanced 5-fold compared to wild-type

• in addition, there is a population of CD8⁺ T cells that are TCR^int/hiCD5^lo/- which are not found in wild-type mice

hematopoietic system

abnormal T cell number ( J:131287 )

• transgene expression rescues the reduced number of CD4⁺ T cells found in the Tox^tm1.1Kay homozygotes

• the percentage of thymic single positive CD8⁺ T cells are enhanced 5-fold compared to wild-type

• in addition, there is a population of CD8⁺ T cells that are TCR^int/hiCD5^lo/- which are not found in wild-type mice

Allelic Composition	Tg(DO11.10)10Dlo/? Tox^tm1.1Kay/Tox^tm1.1Kay
Genetic Background	involves: 129S/Sv * BALB/c * C3H * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
Tox^tm1.1Kay mutation (0 available); any Tox mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

arrested T cell differentiation ( J:131287 )

• transgenic T cell development is arrested after positive selection but before CD4⁺T cell lineage commitment

decreased T cell number ( J:131287 )

• mice exhibit severe lymphopenia with only a minor population of CD8⁺ T cells found in the spleen

hematopoietic system

arrested T cell differentiation ( J:131287 )

• transgenic T cell development is arrested after positive selection but before CD4⁺T cell lineage commitment

decreased T cell number ( J:131287 )

• mice exhibit severe lymphopenia with only a minor population of CD8⁺ T cells found in the spleen

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