Phenotypes associated with this allele

• Allele Symbol: Lrfn2^{Tg(CD2-Myb/en)5Kmw}
• Allele Name: transgene insertion 5, Kathleen Mary Weston
• Allele ID: MGI:3761369
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lrfn2^Tg(CD2-Myb/en)5Kmw/Lrfn2^Tg(CD2-Myb/en)5Kmw	involves: C57BL/10 * CBA	MGI:3761386
ht2	Lrfn2^Tg(CD2-Myb/en)5Kmw/Lrfn2^+	involves: C57BL/10 * CBA	MGI:3761385
cx3	Lrfn2^Tg(CD2-Myb/en)5Kmw/Lrfn2^+ Tg(H2-K-Myc)1Brn/?	involves: C57BL/10 * CBA * FVB/N	MGI:3761491

Genotype
MGI:3761386

hm1

• Allelic Composition: Lrfn2^{Tg(CD2-Myb/en)5Kmw}/Lrfn2^{Tg(CD2-Myb/en)5Kmw}
• Genetic Background: involves: C57BL/10 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:126456 )

• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation

abnormal thymus cortex morphology ( J:126456 )

• thymi display structurally abnormal cortices

abnormal thymus medulla morphology ( J:126456 )

• thymi lack discernible medullas

small thymus ( J:126456 )

• mice have little or no thymus

decreased T cell number ( J:126456 )

• T cell number is reduced by 20-fold

decreased double-negative T cell number ( J:126456 )

• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes

decreased CD8-positive, alpha-beta T cell number ( J:126456 )

• the number of CD8+ single negative by 4-fold compared to in wild-type mice

small spleen ( J:126456 )

• mice have spleens that are between a half to a quarter the size of those in wild-type mice

• mice have little or no thymus

hematopoietic system

decreased T cell proliferation ( J:126456 )

• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation

abnormal thymus cortex morphology ( J:126456 )

• thymi display structurally abnormal cortices

abnormal thymus medulla morphology ( J:126456 )

• thymi lack discernible medullas

small thymus ( J:126456 )

• mice have little or no thymus

decreased T cell number ( J:126456 )

• T cell number is reduced by 20-fold

decreased double-negative T cell number ( J:126456 )

• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes

decreased CD8-positive, alpha-beta T cell number ( J:126456 )

• the number of CD8+ single negative by 4-fold compared to in wild-type mice

small spleen ( J:126456 )

• mice have spleens that are between a half to a quarter the size of those in wild-type mice

• mice have little or no thymus

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:126456 )

• thymi display structurally abnormal cortices

abnormal thymus medulla morphology ( J:126456 )

• thymi lack discernible medullas

small thymus ( J:126456 )

• mice have little or no thymus

cellular

decreased T cell proliferation ( J:126456 )

• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation

Genotype
MGI:3761385

ht2

• Allelic Composition: Lrfn2^{Tg(CD2-Myb/en)5Kmw}/Lrfn2⁺
• Genetic Background: involves: C57BL/10 * CBA

hematopoietic system

decreased T cell proliferation ( J:126456 )

• T cell proliferation induced by 2C11 is reduced by 50%

abnormal bone marrow cell morphology/development ( J:125805 )

• mice exhibit a slight increase in proerythroblasts and basophilic erythroblasts compared to Tg(H2-K-Myc)1Brn Lrfn2^{Tg(CD2-Myb/en)5Kmw} mice

abnormal common myeloid progenitor cell morphology ( J:125805 )

• in a 14 day culture, spleen cells produce 2.5-fold more derived spleen-derived colonies

• the proportion of colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) from cultured spleen cells is increased to 19% compared to 10% for wild-type spleen cells

• in a 14 day culture, bone marrow cells colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies

decreased T cell number ( J:126456 )

• T cell number is reduced by 2- to 4-fold

immune system

decreased T cell proliferation ( J:126456 )

• T cell proliferation induced by 2C11 is reduced by 50%

decreased T cell number ( J:126456 )

• T cell number is reduced by 2- to 4-fold

abnormal lymph node morphology ( J:125805 )

• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes although to a lesser degree than in homozygotes mice exhibit more mature R4 and R5 gate cells than in Tg(H2-K-Myc)1Brn Lrfn2^{Tg(CD2-Myb/en)5Kmw} mice

cellular

decreased T cell proliferation ( J:126456 )

• T cell proliferation induced by 2C11 is reduced by 50%

Genotype
MGI:3761491

cx3

• Allelic Composition: Lrfn2^{Tg(CD2-Myb/en)5Kmw}/Lrfn2⁺; Tg(H2-K-Myc)1Brn/?
• Genetic Background: involves: C57BL/10 * CBA * FVB/N

mortality/aging

premature death ( J:125805 )

• all mice had to be sacrificed at 14 weeks because of their enlarged peripheral lymph nodes

immune system

lymph node hemorrhage ( J:125805 )

• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice

abnormal lymph node morphology ( J:125805 )

• lymph nodes contain large hematomas with cystic degeneration and diffuse hemorrhage with necrosis and become a site of extramedullary hematopoiesis

• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes

• the majority of abnormal cells in the lymph node are orthochromatophilic eryththroblasts with a small amount of R2 and R4 gate cells indicating that all stages of erythroblasts are present

• fewer than 2% of lymph node cells express T cell, B cell or granulocyte markers

lymph node necrosis ( J:125805 )

enlarged lymph nodes ( J:125805 )

• between 4 and 10 weeks all mice exhibit enlarged lymph nodes (up to 1 cm in diameter at 8 weeks of age)

hematopoietic system

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice

abnormal erythropoiesis ( J:125805 )

• both single and double transgenic erythrocytes are recovered

• the lymph nodes and spleen become sites of extramedullary hematopoiesis

extramedullary hematopoiesis ( J:125805 )

• the lymph nodes and spleen become sites of extramedullary hematopoiesis

abnormal bone marrow cell morphology/development ( J:125805 )

• bone marrow contains 3.5% R2 gate cells (proerythroblasts) compared to 1% in wild-type mice and twice the number of R3 gate cells (basophilic erythroblasts)

abnormal common myeloid progenitor cell morphology ( J:125805 )

• in a 14 day culture, bone marrow cells produce twice as many colonies as wild-type and single transgenic cells with a bias towards producing colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies

cardiovascular system

lymph node hemorrhage ( J:125805 )

• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

cellular

lymph node necrosis ( J:125805 )

growth/size/body

enlarged spleen ( J:125805 )

spleen hyperplasia ( J:125805 )

• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x10⁸ and 25x10⁸ with the greatest numbers observed in the older mice