Analysis Tools
mortality/aging
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• embryos die early postimplantation
• however, lethality is partially rescued on a Trp53- background
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Allele Symbol Allele Name Allele ID |
Rad51ctm1.1Sks targeted mutation 1.1, Shyam K Sharan MGI:3707425 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• embryos die early postimplantation
• however, lethality is partially rescued on a Trp53- background
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Testes abnormalities in Rad51ctm1Sks/Rad51ctm1.1Sks mice
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• in infertile females at 12 weeks of age
• however, corpora lutea are present after superovulation of infertile females
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• 60-75% of infertile females oocytes have poorly structured metaphase plates compared to 30% in controls (Rad51ctm1Sks heterozygotes)
• however, chromosomes align normally along the metaphase I plate and there are no apparent defects in pairing and cohesion of chromosomes
• oocytes of hormone-treated infertile females have a variety of chromosome abnormalities at metaphase II including precocious separation of chromatids
• in oocytes with precocious separation of chromatids some chromatids are accentric and a few have two centromeres
• 20% of oocytes from superovulated infertile females have greater than 20 chromosomes and some have 20 or fewer pairs of centromeres
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• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes
• even though testes morphology and cell composition improves with age males are still infertile up to 8 months of age
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• infertile mice have few mature spermatozoa in tubules
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• infertile mice have increased number of apoptotic spermatocytes in the testes
• spermatocyte distribution is shifted towards early stages of prophase I (leptotene and early to mid zygotene) with fewer cells in the late zygotene or pachytene stages
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• infertile mice have impaired spermatogenesis during meiosis I and rarely progress beyond metaphase I
• during meiosis, cells display unpaired autosomal univalents and persistence of double strand breaks
• however, no signs of precocious separation of sister chromatids is detected
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• at 4 weeks of age, tubules are deformed and often almost devoid of germ cells
• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes
• at 12 weeks of age, interstitial tissue appears hypertrophied in both fertile and infertile mice
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• testes of infertile males are smaller and weighed less at 8-10 weeks of age than fertile males
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• at E8.5 fewer embryos are removed from the uterine horns of superovulated infertile females compared to superovulated controls and 7 of 10 of these embryos are developmentally abnormal
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• some males and females are infertile
• however, sexual activity is normal and fertile mice produce normal sized litters compared to controls
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• 11.6% of females are infertile
• however, infertile females injected with pregnant mare serum and human chorionic gonadotropin can be successfully mated
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• 36.6% of males are infertile
• even though testes morphology and cell composition improves with age males are still infertile up to 8 months of age
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• in infertile females at 12 weeks of age
• however, corpora lutea are present after superovulation of infertile females
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• at 4 weeks of age, tubules are deformed and often almost devoid of germ cells
• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes
• at 12 weeks of age, interstitial tissue appears hypertrophied in both fertile and infertile mice
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• testes of infertile males are smaller and weighed less at 8-10 weeks of age than fertile males
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• infertile mice have few mature spermatozoa in tubules
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• infertile mice have increased number of apoptotic spermatocytes in the testes
• spermatocyte distribution is shifted towards early stages of prophase I (leptotene and early to mid zygotene) with fewer cells in the late zygotene or pachytene stages
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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