Phenotypes associated with this allele

• Allele Symbol: Ptgs2^{tm2.1(Ptgs1)Fun}
• Allele Name: targeted mutation 2.1, Colin D Funk
• Allele ID: MGI:3699107
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptgs2^tm2.1(Ptgs1)Fun/Ptgs2^tm2.1(Ptgs1)Fun	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB	MGI:3701212
cx2	Ptgs1^tm1Fun/Ptgs1^tm1Fun Ptgs2^tm2.1(Ptgs1)Fun/Ptgs2^tm2.1(Ptgs1)Fun	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * FVB	MGI:3701213

Genotype
MGI:3701212

hm1

• Allelic Composition: Ptgs2^{tm2.1(Ptgs1)Fun}/Ptgs2^{tm2.1(Ptgs1)Fun}
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

decreased urine prostaglandin level ( J:118171 )

• urinary prostanglandin biosynthesis (eg. prostacyclin production) is decreased by 55% relative to wild-type

kidney inflammation ( J:118171 )

• glomeruli have increase inflammatory cell infiltration

abnormal renal glomerulus morphology ( J:118171 )

• in older mice, glomeruli are enlarged to due increased glomerular cellularity

• cross-sectional area is increased ~56% in mutants compared to wild-type

homeostasis/metabolism

increased blood urea nitrogen level ( J:118171 )

• BUN levels are increased ~2-fold vs wild-type at 6 months of age

decreased urine prostaglandin level ( J:118171 )

• urinary prostanglandin biosynthesis (eg. prostacyclin production) is decreased by 55% relative to wild-type

cardiovascular system

decreased vascular permeability ( J:118171 )

• dye extravasation in the ear vasculature is decreased by 50-60% with bradykinin challenge compared to wild-type

immune system

peritoneal inflammation ( J:118171 )

• at 5 months of age, mice show chronic peritonitis

abnormal macrophage physiology ( J:118171 )

• upon LPS stimulation and 20-30 uM arachadonic acid, induced mutant macrophages produce prostaglandin E2 similar to wild-type levels

• upon LPS stimulation with endognenous or very low exogenous substrate, levels of PGE2 produced by mutant macrophages are much lower than wild-type

kidney inflammation ( J:118171 )

• glomeruli have increase inflammatory cell infiltration

digestive/alimentary system

peritoneal inflammation ( J:118171 )

• at 5 months of age, mice show chronic peritonitis

reproductive system

abnormal fertility/fecundity ( J:118171 )

• females show a longer period between successive pregnancies

decreased litter size ( J:118171 )

• litter size is somewhat reduced compared to wild-type

hematopoietic system

abnormal macrophage physiology ( J:118171 )

• upon LPS stimulation and 20-30 uM arachadonic acid, induced mutant macrophages produce prostaglandin E2 similar to wild-type levels

• upon LPS stimulation with endognenous or very low exogenous substrate, levels of PGE2 produced by mutant macrophages are much lower than wild-type

Genotype
MGI:3701213

cx2

• Allelic Composition: Ptgs1^tm1Fun/Ptgs1^tm1Fun; Ptgs2^{tm2.1(Ptgs1)Fun}/Ptgs2^{tm2.1(Ptgs1)Fun}
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * FVB

mortality/aging

prenatal lethality ( J:118171 )

• no homozygous offspring are produced from double heterozygous matings