About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Lcktm1Dbs
targeted mutation 1, David B Straus
MGI:3690319
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Lcktm1Dbs/Lcktm1Dbs involves: C3H * C57BL/6 MGI:3698306
cx2
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)1100Mjb/0 		involves: C3H * C57BL/6 MGI:3698307
cx3
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)425Cbn/0 		involves: C3H * C57BL/6 MGI:3698308
cx4
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraH-Y,TcrbH-Y)71Vbo/0 		involves: C3H * C57BL/6 * DBA/2J MGI:3698309


Genotype
MGI:3698306
hm1
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced
abnormal T cell differentiation ( J:114660 )
• T cell development is altered as mutants have ~50% of thymocyte number of wild-type
abnormal double-negative T cell morphology ( J:114660 )
• relative accumulation of CD25+CD44- thymocytes (double negative stage 3, DN3) compared to wild-type
• DN3/DN4 ratio is 10 in mutants vs ~2 in controls
decreased T cell number ( J:114660 )
• reduced numbers of T cells in lymph nodes and spleen
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type
abnormal CD4-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal
abnormal CD8-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal

immune system
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced
abnormal T cell differentiation ( J:114660 )
• T cell development is altered as mutants have ~50% of thymocyte number of wild-type
abnormal double-negative T cell morphology ( J:114660 )
• relative accumulation of CD25+CD44- thymocytes (double negative stage 3, DN3) compared to wild-type
• DN3/DN4 ratio is 10 in mutants vs ~2 in controls
decreased T cell number ( J:114660 )
• reduced numbers of T cells in lymph nodes and spleen
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type
abnormal CD4-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal
abnormal CD8-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal

endocrine/exocrine glands
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type

cellular
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced




Genotype
MGI:3698307
cx2
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (93 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is diminished

immune system
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is diminished

cellular
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature




Genotype
MGI:3698308
cx3
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (93 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is compromised
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed

immune system
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is compromised
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed




Genotype
MGI:3698309
cx4
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic
Background		 involves: C3H * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (93 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal positive T cell selection ( J:114660 )
• almost complete block of positive selection is observed
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed

immune system
abnormal positive T cell selection ( J:114660 )
• almost complete block of positive selection is observed
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory