Phenotypes associated with this allele
cellular
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• primary mouse embryonic fibroblasts exposed to 4OHT for 5 days to activate cre/ERT2 recombinase exhibit decreased proliferation that is similar to that in single Kras mutants without further additive effects
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normal phenotype
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• 30 day old mice fed a tamoxifen diet for 3 months are healthy and show normal weight and activity and no obvious anatomical defects
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Raf1tm2Bacc mutation
(0 available);
any
Raf1 mutation
(114 available)
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nervous system
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• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons
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cellular
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• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation
(0 available);
any
Braf mutation
(58 available)
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Raf1tm1Bacc mutation
(0 available);
any
Raf1 mutation
(114 available)
Raf1tm2Bacc mutation
(0 available);
any
Raf1 mutation
(114 available)
Tg(Nes-cre)1Kln mutation
(4 available)
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mortality/aging
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• no double conditional embryos are found live at E14-15, but can be isolated at E13
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nervous system
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• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
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• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
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• at E13, Ret levels in DRGs are reduced
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cellular
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• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Raf1tm2Bacc mutation
(0 available);
any
Raf1 mutation
(114 available)
Tg(Nes-cre)1Kln mutation
(4 available)
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mortality/aging
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• most die before weaning
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growth/size/body
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• mice are smaller in the postnatal period than Braf conditional knockouts
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nervous system
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• deficiency of terminal axonal projections of parvalbumin-positive neurons toward the lateral motor pools of the spinal cord is seen compared to controls
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• proprioceptive axons enter spinal cord normally, but few progress beyond intermediate zone
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• at P12, number of Ret+ neurons in DRG is reduced; numbers of CGRG+ neurons are increased
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Raf1tm2Bacc mutation
(0 available);
any
Raf1 mutation
(114 available)
Tg(Nes-cre)1Kln mutation
(4 available)
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normal phenotype
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• mice show no phenotypic abnormalities at any stage
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mortality/aging
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• although born alive death occurs around 21 days of age from aggressive neurodegenerative disease
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growth/size/body
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• after being born alive, pups show progressive growth retardation
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Tg(Tek-cre/ERT2)1Arnd mutation
(1 available)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Tg(Camk2a-cre)1557Sva mutation
(0 available)
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behavior/neurological
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• six month old mice have poor context discrimination although contextual conditioning is normal
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• six month old mice much slower to learn the platform position in a hidden platform Morris water maze (Motor coordination normal)
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nervous system
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• six month old mice are deficient in the early phase of long term potentiation although there is no disruption of synaptic transmission or presynaptic plasticity
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mortality/aging
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• following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre), mice show 50% survival at 41.5 weeks compared to 40 weeks in single Kras heterozygous controls, indicating similar survival
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neoplasm
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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respiratory system
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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cellular
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• primary mouse embryonic fibroblasts exposed to 4OHT for 5 days to activate cre/ERT2 recombinase exhibit decreased proliferation compared to single Kras heterozygotes
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation
(0 available);
any
Braf mutation
(58 available)
Braftm1Sva mutation
(1 available);
any
Braf mutation
(58 available)
Tg(Nes-cre)1Kln mutation
(4 available)
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mortality/aging
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• mice tend to die in third week postnatal, although some survive to P35
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growth/size/body
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• at P32, body weight is ~25% of littermates
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• from P12-14 onwards, mice show severe growth retardation
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behavior/neurological
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N |
• mice show normal nociceptive response in hotplate assay
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• mice appear hyperactive
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nervous system
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N |
• dorsal root ganglion neurons survive in mutants
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• perivascular hypothalamic axonal innervation is reduced
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• anterior lobe of pituitary gland is markedly reduced in size compared to controls
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• disproportionate thinning of cortex is observed
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endocrine/exocrine glands
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• perivascular hypothalamic axonal innervation is reduced
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• anterior lobe of pituitary gland is markedly reduced in size compared to controls
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homeostasis/metabolism
Analysis Tools