Phenotypes associated with this allele

• Allele Symbol: Tg(KRT14-Kitl)1Takk
• Allele Name: transgene insertion 1, Takahiro Kunisada
• Allele ID: MGI:3607790
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Mc1r^e/Mc1r^e Xpc^tm1Brd/Xpc^tm1Brd Tg(KRT14-Kitl)1Takk/0	involves: 129S7/SvEvBrd * C57BL/6 * SJL	MGI:3686788
cx2	Xpa^tm1Tnka/Xpa^tm1Tnka Tg(KRT14-Kitl)1Takk/?	involves: C57BL/6 * CBA * SJL	MGI:4361120
cx3	Mc1r^e/Mc1r^e Tg(KRT14-Kitl)1Takk/0	involves: C57BL/6 * SJL	MGI:3686789

Genotype
MGI:3686788

cx1

• Allelic Composition: Mc1r^e/Mc1r^e; Xpc^tm1Brd/Xpc^tm1Brd; Tg(KRT14-Kitl)1Takk/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

weight loss ( J:112959 )

• vehicle-treated mutants show weight loss, indicative of failure to thrive, compared to forskolin-treated animals

neoplasm

increased tumor incidence ( J:112959 )

• vehicle-treated mutant controls develop tumors more rapidly (11 tumors ~4 weeks after 20 weeks of UV-B exposure) compared to forskolin-treated mice (6 tumors, onset ~25 weeks)

• other types of tumors are found in vehicle-treated mice (3/11); 2/9 mice develop multiple tumors

increased squamous cell carcinoma incidence ( J:112959 )

• tumors in vehicle-treated mutants are mainly this type (6/11) while forskolin-treated mice develop only this type

integument

thick epidermis ( J:112959 )

• vehicle-treated mutants show significant sun damage after 20 weeks of UV-B exposure including profound epidermal thickening while forskoling treatment prevented damage

skin lesions ( J:112959 )

• vehicle-treated mutants show evidence of sun damage like scarring after 20 weeks of UV-B exposure while forskolin treatment prevented damage

Genotype
MGI:4361120

cx2

• Allelic Composition: Xpa^tm1Tnka/Xpa^tm1Tnka; Tg(KRT14-Kitl)1Takk/?
• Genetic Background: involves: C57BL/6 * CBA * SJL

pigmentation

abnormal coat/hair pigmentation ( J:100608 )

• black hair

abnormal epidermal pigmentation ( J:100608 )

• black skin

neoplasm

increased skin tumor incidence ( J:100608 )

• mice irradiated with UV-B 3X/week for 10 weeks, total dose 150J/cm²

• unevenly pigmented macules appear on re-epithelialized skin at 4 months after UV-B exposure

• lentigo maligna melanomas eventually become elevated tumors

• black nodular melanomas in some mice by 6 months after exposure

• at 53 weeks, 8 of 61 mice with nodular melanomas and 12 of 61 mice show lintigo maligna melanoma

• one type of tumor or the other, never both

increased metastatic potential ( J:100608 )

• melanoma cells invade the dermis

• both types of melanomas become metastatic

integument

abnormal coat/hair pigmentation ( J:100608 )

• black hair

abnormal epidermal pigmentation ( J:100608 )

• black skin

increased skin tumor incidence ( J:100608 )

• mice irradiated with UV-B 3X/week for 10 weeks, total dose 150J/cm²

• unevenly pigmented macules appear on re-epithelialized skin at 4 months after UV-B exposure

• lentigo maligna melanomas eventually become elevated tumors

• black nodular melanomas in some mice by 6 months after exposure

• at 53 weeks, 8 of 61 mice with nodular melanomas and 12 of 61 mice show lintigo maligna melanoma

• one type of tumor or the other, never both

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
skin melanoma		DOID:8923	OMIM:608035 OMIM:612263	J:100608

Genotype
MGI:3686789

cx3

• Allelic Composition: Mc1r^e/Mc1r^e; Tg(KRT14-Kitl)1Takk/0
• Genetic Background: involves: C57BL/6 * SJL

pigmentation

abnormal melanogenesis ( J:112959 )

• in mutants, no measurable UV-induced melanization is observed; forskolin application to the skin induces significant skin darkening