About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Scn9atm1Dgen
targeted mutation 1, Deltagen
MGI:3604573
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Scn9atm1Dgen/Scn9atm1Dgen B6.129P2-Scn9atm1Dgen/J MGI:5790336
hm2
Scn9atm1Dgen/Scn9atm1Dgen C.129P2(B6)-Scn9atm1Dgen MGI:5790340
hm3
Scn9atm1Dgen/Scn9atm1Dgen either: (involves: 129P2/OlaHsd * BALB/c * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * CD-1) MGI:5790338
hm4
Scn9atm1Dgen/Scn9atm1Dgen involves: 129P2/OlaHsd * C57BL/6 MGI:3606595
ht5
Scn9atm1Dgen/Scn9a+ involves: 129P2/OlaHsd * C57BL/6 MGI:3606596


Genotype
MGI:5790336
hm1
Allelic
Composition
Scn9atm1Dgen/Scn9atm1Dgen
Genetic
Background
B6.129P2-Scn9atm1Dgen/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation (1 available); any Scn9a mutation (68 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• Background Sensitivity: no viable mice are obtained on a congenic C57BL/6 background, indicating neonatal lethality, compared to 20% survival on mixed backgrounds




Genotype
MGI:5790340
hm2
Allelic
Composition
Scn9atm1Dgen/Scn9atm1Dgen
Genetic
Background
C.129P2(B6)-Scn9atm1Dgen
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation (1 available); any Scn9a mutation (68 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• Background Sensitivity: nurturing of neonates on the congenic BALB/c background does not improve survival as it does on a mixed backgrounds




Genotype
MGI:5790338
hm3
Allelic
Composition
Scn9atm1Dgen/Scn9atm1Dgen
Genetic
Background
either: (involves: 129P2/OlaHsd * BALB/c * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation (1 available); any Scn9a mutation (68 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• Background Sensitivity: most mice on a mixed BALB/c background die before P14 while most mice on a mixed CD-1 background die before P20 compared to complete neonatal lethality of mice on a congenic C57BL/6 or BALB/c background
• Background Sensitivity: mice on either a mixed BALB/c or CD-1 background show an approximate 20% survival rate to adulthood when neonates are nurtured (by hand feeding, by receiving supplementary dextrose injections when dehydrated, and by culling litters to prevent competition for resources)

growth/size/body
• neonates are smaller than wild-type mice, however mice that survive to adulthood show normal body size

behavior/neurological
• in the tail-clip assay, mutants are insensitive to mechanical hyperalgesia and do not react to a clip placed on the tail
• however, mice show unchanged tactile sensitivity measured with von Frey fibers
• mice lack a response to histamine, showing few to no scratching bouts in response to histamine injection, although a classic flare response at the injection site is seen
• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls
• mice do not respond to nociceptive stimuli
• however, mice exhibit normal overall locomotion and rearing
• mice exhibit reduced responses in both phases of the formalin pain assay, with licking and lifting in phase I of the formalin response being about 80% less in mutants and little to no licking and lifting was seen in phase II
• mice treated with veratradine or grayanotoxin III show reduced lifting and licking response, with an 85% and 96% lower response, respectively, than controls indicating that mice are insensitive to pain resulting from activation of peripheral sodium channels
• in the hot plate test, latency to withdrawal at each temperature is longer in mutants and mice do not display painful behaviors
• in the Hargreaves apparatus, mutant mice react with an approximate 40% increase in latency time compared to controls
• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls

immune system
• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis

integument
• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis

nervous system
• C-fiber spontaneous action potential frequency is 6-fold lower in saphenous nerve
• C-fiber action potential frequency is reduced in response to mechanical stimuli at the higher spiking rates evoked by stronger stimulus
• mice show reduced tetrodotoxin-sensitive sodium current recorded from sensory neuron cell bodies

renal/urinary system
• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, some mice, mainly males, present with urinary issues such as prolapses and blockage

taste/olfaction
• in a buried food assay, 18 of 19 mice do not find the hidden food pellet




Genotype
MGI:3606595
hm4
Allelic
Composition
Scn9atm1Dgen/Scn9atm1Dgen
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation (1 available); any Scn9a mutation (68 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• grossly normal homozygous embryos are identified at E12.5 to E18.5, but not at later stages




Genotype
MGI:3606596
ht5
Allelic
Composition
Scn9atm1Dgen/Scn9a+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation (1 available); any Scn9a mutation (68 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• exhibit significantly increased prepulse inhibition compared with age- and gender-matched wildtype controls





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/19/2021
MGI 6.17
The Jackson Laboratory