Analysis Tools
normal phenotype
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• no notable abnormalities in the hematopoietic system were detected
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Allele Symbol Allele Name Allele ID |
Cebpatm1Dgt targeted mutation 1, Daniel G Tenen MGI:3522010 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no notable abnormalities in the hematopoietic system were detected
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• although born in Mendelian ratios, most mice exposed to doxycycline from conception die of respiratory failure at birth
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• doxycycline-exposed newborn mice show a reduction of airspace, sometimes obliterating the alveolar structure
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• in addition, neonatal alveolar cells show significantly decreased numbers of TUNEL-positive cells, indicating reduced apoptosis
• doxycycline-exposed newborn mice display increased numbers of Ki-67-positive alveolar cells, indicating increased alveolar cell proliferation
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• doxycycline-exposed newborn mice lack type I alveolar cells
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• alveoli of doxycycline-exposed newborn mice are lined by immature cuboidal type II cells with a clear cytosol, round nuclei and high glycogen content, indicating a type II cell differentiation arrest
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• immature type II alveolar cells of doxycycline-exposed newborn mice lack lamellar bodies
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• doxycycline-exposed newborn mice lack mature type II alveolar cells
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• doxycycline-exposed newborn mice display thicker alveolar walls due to marked cellular accumulation
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• 86% of mice exposed to doxycycline from conception show signs of respiratory distress within hours after birth
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• most mice exposed to doxycycline from conception to birth display respiratory failure
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• doxycycline-exposed newborn mice show a marked loss of surfactant protein B (SP-B) by immunostaining
• mRNAs for SP-A and SP-D are significantly down-regulated
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| N |
• doxycycline-exposed mice have a normal liver architecture and show normal Cebpa mRNA levels in liver
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| N |
• doxycycline-exposed mice exhibit normal granulocytic differentiation in fetal liver and contain granulocytes in peripheral blood
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mutants treated with poly I:C rarely survive beyond 4-5 weeks of age due to granulocytopenia and sepsis
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• a dramatic loss of Gr-1+ Mac-1+ neutrophils/monocytes is seen in the bone marrow, spleen, and peripheral blood, however T and B cell numbers are normal
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• after poly I:C treatment myelocytes, neutrophils, monocytes, and eosinophils are rarely seen in the bone marrow and the number of immature myeloblasts is increased 32%
• in vitro common myeloid progenitor cells fail to form any mature granulocyte-macrophage, macrophage, or granulocytic colonies
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• injection of poly I:C at 2 days after birth resulted in absence of mature granulocytes, however mutants do not develop anemia, thrombocytopenia, or leukemia
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• a dramatic loss of Gr-1+ Mac-1+ neutrophils/monocytes is seen in the bone marrow, spleen, and peripheral blood, however T and B cell numbers are normal
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• after poly I:C treatment myelocytes, neutrophils, monocytes, and eosinophils are rarely seen in the bone marrow and the number of immature myeloblasts is increased 32%
• in vitro common myeloid progenitor cells fail to form any mature granulocyte-macrophage, macrophage, or granulocytic colonies
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• injection of poly I:C at 2 days after birth resulted in absence of mature granulocytes, however mutants do not develop anemia, thrombocytopenia, or leukemia
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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