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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(TcraTcrb)8Rest
transgene insertion 8, Nicholas Restifo
MGI:3051150
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Pgdtm1.1Pse/Pgdtm1.1Pse
Tg(Cd4-cre)1Cwi/0
Tg(TcraTcrb)8Rest/0
involves: C57BL/6 * DBA/2 MGI:6751656
cx2
Ctla4tm1All/Ctla4tm1All
Rag1tm1Mom/Rag1tm1Mom
Tg(TcraTcrb)8Rest/0
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6 MGI:4940117
cx3
Ctla4tm1All/Ctla4tm1All
Tg(TcraTcrb)8Rest/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:4940116
tg4
Tg(TcraTcrb)8Rest/? C57BL/6-Tg(TcraTcrb)8Rest MGI:3789128


Genotype
MGI:6751656
cn1
Allelic
Composition
Pgdtm1.1Pse/Pgdtm1.1Pse
Tg(Cd4-cre)1Cwi/0
Tg(TcraTcrb)8Rest/0
Genetic
Background
involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pgdtm1.1Pse mutation (0 available); any Pgd mutation (15 available)
Tg(Cd4-cre)1Cwi mutation (8 available)
Tg(TcraTcrb)8Rest mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• CD8+ T cells co-cultured with B16-F10 melanoma cells uptake glucose more effectively than control cells
• adaptive transfer of mutant CD8+ T cells to congenic recipients bearing the B16-F10 melanoma results in smaller tumor growth than in controls

hematopoietic system
• CD8+ T cells co-cultured with B16-F10 melanoma cells uptake glucose more effectively than control cells
• adaptive transfer of mutant CD8+ T cells to congenic recipients bearing the B16-F10 melanoma results in smaller tumor growth than in controls




Genotype
MGI:4940117
cx2
Allelic
Composition
Ctla4tm1All/Ctla4tm1All
Rag1tm1Mom/Rag1tm1Mom
Tg(TcraTcrb)8Rest/0
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4tm1All mutation (1 available); any Ctla4 mutation (34 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (89 available)
Tg(TcraTcrb)8Rest mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• mice exhibit a normal lifespan

immune system
N
• CD8+ T cells exhibit a completely naive phenotype

pigmentation
N
• mice do not develop autoimmune vitiligo




Genotype
MGI:4940116
cx3
Allelic
Composition
Ctla4tm1All/Ctla4tm1All
Tg(TcraTcrb)8Rest/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4tm1All mutation (1 available); any Ctla4 mutation (34 available)
Tg(TcraTcrb)8Rest mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die at 10 to 12 weeks

immune system
• hgp100(25-33)-treated splenocyte exhibit increased IFN-gamma secretion compared with splenocytes from Tg(TcraTcrb)8Rest mice

pigmentation
• at 4 to 6 weeks, 48 of 49 mice develop autoimmune vitiligo unlike Tg(TcraTcrb)8Rest mice

hematopoietic system

integument
• at 4 to 6 weeks, 48 of 49 mice develop autoimmune vitiligo unlike Tg(TcraTcrb)8Rest mice




Genotype
MGI:3789128
tg4
Allelic
Composition
Tg(TcraTcrb)8Rest/?
Genetic
Background
C57BL/6-Tg(TcraTcrb)8Rest
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraTcrb)8Rest mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• over 95 % of the CD8 T cells present in circulation express the transgenic TCR that is specific for the H2-Db restricted epitope of Silv (gp100), a protein that is highly expressed in melanoma cells
• these transgenic T cells constitute about 20% of spleen cells
• the baseline levels of activation markers for the transgenic T cells indicate that most of cells are in a nave state
• upon in vitro stimulation with the immunogenic epitope of gp100, CD8 T cells are activated and proliferate extensively
• CD 8 T cells produce large amounts of IFN-gamma when cultured in the presence of melanoma cells
• large number of IFN-gamma producing CD8 T cells are found in melanoma tumors that have regressed due to treatment with gp100 vaccine, IL-2, and adoptive transfer of transgenic CD8 T cells

neoplasm
• despite the presence of large number of anti-gp100 CD8 T cells, transplanted melanoma tumors that express gp100 have the same growth kinetics as tumors transplanted into control mice
• adoptive transfer of transgenic splenocytes into normal mice bearing melanoma tumors also has no effect on tumor growth
• there is a modest delay in tumor growth when mice that are recipients of transgenic T cells are also vaccinated with peptides that mimic the gp100 epitope
• when IL-2 is administered with the vaccine, there is a large regression in tumor size in mice that are also recipients of transgenic T cells
• mice bearing melanoma tumors that receive vaccine, transgenic T cells, and IL-2 have survival times of over 1 year compared to controls that die within 2 months

hematopoietic system
• over 95 % of the CD8 T cells present in circulation express the transgenic TCR that is specific for the H2-Db restricted epitope of Silv (gp100), a protein that is highly expressed in melanoma cells
• these transgenic T cells constitute about 20% of spleen cells
• the baseline levels of activation markers for the transgenic T cells indicate that most of cells are in a nave state
• upon in vitro stimulation with the immunogenic epitope of gp100, CD8 T cells are activated and proliferate extensively





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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory