mortality/aging
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• a large portion of embryos are resorbed after E10, though some mice develop normally and survive to adulthood
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cardiovascular system
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N |
• mice that survive to adulthood exhibit normal platelet number and function, coagulation parameters, basal heart rate, and arterial blood pressure
(J:38032)
• mutants exhibit normal pulmonary vasoreactivity to vasoactive mediators
(J:124465)
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• cell density is greater in normal carotids from mutants than in wild-type mice
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• mutants exhibit a reduction of the hypertensive response to the selective thrombin receptor activating peptide, SFLLRN-NH2, before and after L-NAME (an inhibitor of nitric oxide synthesis) and absence of hypertension in the presence of L-NAME
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• mutants show absence of an arterial pressure response to the selective thrombin receptor activating peptide, TFLLRNPNDK-NH2
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• the hypertensive response and heart rate decrease in response to TFLLRNPNDK, a F2r (PAR-1) selective activating peptide, is absent
• SFLLRN, a nonselective receptor activating peptide, was able to induce hypotension in mutants as in wild-type mice but the heart rate decrease and secondary hypotension following L-NAME are absent
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• the thrombin-induced increase in pulmonary microvessel permeability seen in controls is absent in mutants
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• vessel diameter is unchanged and lumen diameter decreases following vascular injury unlike in wild-type mice in which vessel and lumen diameters increase following injury
• area of neointima formation following vascular injury is slightly less than in wild-type mice
• however, medial cell loss, incidence of thrombosis, endothelial regrowth, and medial thickening after injury are similar to wild-type
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reproductive system
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• matings between homozygous mice occur infrequently and produce less than 3 offspring
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homeostasis/metabolism
digestive/alimentary system
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• mutants have a lower baseline short-circuit current in the colon than wild-type mice but no difference in the short-circuit current response to electrical field stimulation, indicating abnormal basal ion secretion in the colon
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• trinitrobenzene sulfonic acid (TNBS) induced colitis is less severe in mutants than in wild-type mice
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immune system
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• absence of human trypsin IV and rat p23 induced edema and granulocyte infiltration after intraplantar injection
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• trinitrobenzene sulfonic acid (TNBS) induced colitis is less severe in mutants than in wild-type mice
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