• T lymphocyte development normal in the thymus (J:87123)

• Ifn-gamma levels were normal to slightly increased

• Il-2 production defective

• Il-4 production very low

• Il-10 levels normal

• Il-13 levels reduced

• lymph nodes were small in immunized mice

• about 1/2 as many cells/node as in wild-type mice

• production of IgE reduced

• reduction of IgG1 levels

• defect indicated by low Il-4 levels and low IgG1 and IgE levels

• Th2 differentiation normal but function disrupted

• greater succeptibility to experimental autoimmune encephalitis

• Ifn-gamma levels were normal to slightly increased

• Il-2 production defective

• Il-4 production very low

• Il-10 levels normal

• Il-13 levels reduced

• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce less IL-4, IL-17a and interferon gamma than wild-type in response to KLH restimulation

• development of lymphocytes is normal (J:131529)

• only a few mice develop marginal levels of inflammation of the islets

• the number of CD25+ and FoxP3+ CD4 T cells in the periphery and pancreatic draining lymph nodes is decreased 30% compared to in heterozygotes

• the frequency of IFN-gamma+ T cells is decreased compared to in heterozygous mice

• the frequency of IFN-gamma+ T cells is decreased compared to in heterozygous mice

• after 60 weeks, no mice develop diabetes compared with 65% of heterozygotes and 75% in wild-type NOD mice

• anti-GAD65 and anti-insulin antibodies are decreased 3- to 5-fold compared to in heterozygotes

• decreased 3- to 5-fold compared to in heterozygotes

• mice exhibit normal numbers of pancreatic islets (J:131529)

• only a few mice develop marginal levels of inflammation of the islets

• the number of CD25+ and FoxP3+ CD4 T cells in the periphery and pancreatic draining lymph nodes is decreased 30% compared to in heterozygotes

• the frequency of IFN-gamma+ T cells is decreased compared to in heterozygous mice

• severe

• severe

• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce no IL-4, IL-17a or interferon gamma in response to KLH restimulation

• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce no IL-4, IL-17a or interferon gamma in response to KLH restimulation

• naive CD4 T cells cultured with OVA peptide-loaded wild-type splenic antigen presenting cells (APCs) and restimulated with anti-CD3 and secrete less IL-4 and IL-17a and more interferon gamma (Ifng) than stimulated wild-type CD4 T cells

• CD4 T cells cultured with OVA peptide-loaded spleen cells from B7 (CD80/86) deficient mice then restimulated with anti-CD3 do not produce IL-4, IL-17a or Ifng

• T cells activated for 4 days with anti-CD3 and wild-type antigen presenting cells produce more IL-4 than controls but production of IL-17a and interferon gamma is much lower

• tumor incidence (about 20%) is reduced compared to Rc3h1 heterozygotes (more than 50%)

• 5 of 25 double mutants develop T cell lymphoma