All Phenotypes Mapt<tm1(EGFP)Klt> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt	B6.129S4-Mapt^tm1(EGFP)Klt	MGI:5604725
hm2	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt	involves: 129S4/SvJae * C57BL/6	MGI:2670770
cx3	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0	B6.Cg-Tg(MAPT)8cPdav Mapt^tm1(EGFP)Klt/J	MGI:5008417
cx4	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(Thy1-MAPT*)30Schd/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:4940110
cx5	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/?	involves: 129S4/SvJae * C57BL/6 * Swiss Webster	MGI:3663749

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
Genetic Background	B6.129S4-Mapt^tm1(EGFP)Klt

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (428 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:213115 )

• 6 month old male mice freeze less during contextual fear conditioning as compared to wild-type

impaired cued conditioning behavior ( J:213115 )

• 6 month old male mice freeze less during cued fear conditioning as compared to wild-type

abnormal motor learning ( J:213115 )

• no improvement in motor skills or learning is observed in 6 month old male mice on accelerating rotarod as compared to wild-type

• however, latency to fall is similar to wild-type

enhanced spatial learning ( J:213115 )

• improved performance in spatial navigation (escape latency and path length)is observed in 6 month old male mice, but only in the second week (days 6-10) of Morris water maze testing

nervous system

reduced long-term potentiation ( J:213115 )

• mice display a brief episode of potentiation but fail to develop long term potentiation following 3 repetitive theta bursts

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (428 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:66561 )

• homozygotes are viable and fertile with no detectable abnormalities in morphology of the central or peripheral nervous systems

behavior/neurological

behavior/neurological phenotype ( J:169074 )

N	• mice show no significant motor deficits up to 12 months of age • spatial working memory is not impaired

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0
Genetic Background	B6.Cg-Tg(MAPT)8cPdav Mapt^tm1(EGFP)Klt/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (428 available)
Tg(MAPT)8cPdav mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Beta-amyloid plaques in Tg(APPswe,PSEN1dE9)85Dbo/0 mice and neurofibrillary tangles in Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0 mice

behavior/neurological

abnormal spatial learning ( J:172426 )

• 6 month old mutants exhibit slower visuospatial learning than control mice

• in the visuospatial re-learning test performed at 7, 8, 9, and 15 months of age, mutants exhibit a decrease in the speed of re-learning the task compared to controls

• however, mutants perform better than Tg(APPswe,PSEN1dE9)85Dbo mice on the visuospatial re-learning test at 9 and 18 months of age

taste/olfaction

taste/olfaction phenotype ( J:172426 )

N	• mutants do not exhibit Alzheimer's disease-related impairment in olfactory performance in tests based on an operant conditioning procedure and in tests based on a habituation/dishabituation procedure

nervous system

neurofibrillary tangles ( J:172426 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:172426

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(Thy1-MAPT*)30Schd/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (428 available)
Tg(Thy1-MAPT*)30Schd mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:169074 )

• mice show reduced survival relative to wild-type, with almost 100% mortality observed by 16 months

• accelerated mortality is observed before 3 months of age (after 1 month of age) compared to wild-type or Tg(Thy1-MAPT*)30Schd single mutant animals

nervous system

nervous system phenotype ( J:169074 )

N	• at 12 months, neuronal cell numbers are similar in the hippocampal CA1-CA3 sectors and spinal cord to those in Tg(Thy1-MAPT*)30Schd mice; volumes of brain, cortex, hippocampal formation and cervical spinal cord are similar

abnormal brain morphology ( J:169074 )

• mice show significantly increased proportion of insoluble tau protein relative to sarkosyl-soluble tau compared to Tg(Thy1-MAPT*)30Schd single mutants

• soluble tau shows decreased phosphorylation compared to single transgenics, while insoluble tau displays significantly elevated phosporylation levels

• GSK-3 kinase activation in the brain is increased compared to wild-type and single mutants

neurofibrillary tangles ( J:169074 )

• NFTs are detected in the hippocampus, cortex, subcortical areas, brainstem, and spinal cord at 12 months, while no tangles are found in wild-type or Mapt mutants

• density (number) of NFTs is significantly increased in the hippocampus (subiculum and CA1 region) compared to Tg(Thy1-MAPT*)30Schd single mutants, but is similar between lines in the spinal cord

abnormal sciatic nerve morphology ( J:169074 )

• mice display axonal loss and reduction in cross-sectional area and axon density in the sciatic nerve compared to wild-type or Mapt mutants

abnormal spinal cord morphology ( J:169074 )

• mice develop numerous pericaryal rounded inclusions in the spinal cord; these are composed to abnormal filaments mixed with abundant neurofilaments; these are more numerous than seen in single transgenics

behavior/neurological

behavior/neurological phenotype ( J:169074 )

N	• in Y-maze tests, percentage of alternations observed is not significantly different from single transgenics, Mapt mutants or wild-type at 3-6 months of age

impaired coordination ( J:169074 )

• mice show a progressive motor deficit, significant from 6 to 12 months compared to wild-type or Mapt mutants; impairment is much more severe than in Tg(Thy1-MAPT*)30Schd single mutants

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

tau protein deposits ( J:84638 )

• phosphorylated tau accumulates in neuronal cell bodies and dendrites of the hippocampus and neocortex as early as 3 months of age

• in particular, accumulations occur in entorhinal cortex, ventromedial hypothalamus, medial septum and the nucleus of the horizontal limb of the diagonal band

• increase in tau phosphorylation occurs at serine 202, threonine 231 and serine 235 as determined by immunoblot

• tau aggregates in the proximal dendrites have an average width of 15 nm and are not densely packed

• insoluble tau is present in both 2 and 9 month old mice

• paired helical filaments are observed in 9, 12 and 14 month old mice

abnormal neuron morphology ( J:84638 )

• cells in the cortex and hippocampus appear irregularly shaped, often with distorted processes in 13 month old mice

hematopoietic system

abnormal spleen red pulp morphology ( J:174270 )

• splenic red pulp is largely obliterated

abnormal spleen B cell follicle morphology ( J:174270 )

• splenic white pulp contains follicles that are larger and more irregular in shape than normal follicles and are often fused with adjacent follicles

abnormal spleen B cell follicle shape ( J:174270 )

• irregular in shape

increased spleen B cell follicle size ( J:174270 )

immune system

abnormal spleen red pulp morphology ( J:174270 )

• splenic red pulp is largely obliterated

abnormal spleen B cell follicle morphology ( J:174270 )

• splenic white pulp contains follicles that are larger and more irregular in shape than normal follicles and are often fused with adjacent follicles

abnormal spleen B cell follicle shape ( J:174270 )

• irregular in shape

increased spleen B cell follicle size ( J:174270 )

renal/urinary system

abnormal renal glomerulus morphology ( J:174270 )

• kidney glomeruli are dilated probably due to obstruction from the amyloid in the lumen or walls of medullary tubules especially in the papilla

neoplasm

increased lymphoma incidence ( J:174270 )

• malignant lymphoma in the spleen and lymph nodes are seen in some mutants

• megakaryocytes are numerous is some spleens, suggesting a relation to megakaryocytic leukemia

increased follicular lymphoma incidence ( J:174270 )

• splenic architecture is replaced by proliferated immature mononuclear cells arranged in follicular aggregates indicating follicular lymphoma

homeostasis/metabolism

amyloidosis ( J:174270 )

• some mutants older than 18 months exhibit amyloid deposits in the spleen, kidneys, liver, ovary and/or heart

• amyloid is deposited concentrically in the perifollicular sheath, partly or completely encircling the follicles in the spleen

• in the liver, amyloid deposit is in the walls of sinusoids or central veins

• in the kidney, amyloid deposits are seen in the glomeruli

• amyloid deposits in the heart are not composed of amyloid-beta peptide, but rather amyloid A

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:84638 , J:174270

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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