Phenotypes associated with this allele

• Allele Symbol: Blnk^tm1Dkit
• Allele Name: targeted mutation 1, Daisuke Kitamura
• Allele ID: MGI:2665856
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Blnk^tm1Dkit/Blnk^tm1Dkit	involves: C57BL/6	MGI:2665859
cx2	Blnk^tm1Dkit/Blnk^tm1Dkit Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn	involves: 129P2/OlaHsd * C57BL/6 * NZB	MGI:2665858
cx3	Blnk^tm1Dkit/Blnk^tm1Dkit Prkcd^tm1Kin/Prkcd^tm1Kin	involves: 129P2/OlaHsd * C57BL/6 * NZB	MGI:3841693
cx4	Blnk^tm1Dkit/Blnk^tm1Dkit Igh-J^tm1(3H9-VDJ)Mwg/Igh-J^+	involves: C57BL/6 * NZB	MGI:3513975
cx5	Blnk^tm1Dkit/Blnk^tm1Dkit Igh-J^tm1(3H9-VDJ)Mwg/Igh-J^+ Igk-J^tm1Mwg/Igk-J^+	involves: C57BL/6 * NZB	MGI:3513981

Genotype
MGI:2665859

hm1

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased leukemia incidence ( J:84040 )

• pre-B cell leukemia

hematopoietic system

abnormal B cell apoptosis ( J:143854 )

• spontaneous apoptosis is not inhibited by the addition of BAFF unlike in wild-type cells

increased B cell apoptosis ( J:143854 )

abnormal B cell differentiation ( J:84040 )

• B cell development is partially arrested at the B220+ pre-BCR+ (putative large pre-B) cell stage

abnormal immature B cell morphology ( J:94297 )

• proportion of lamda + B cells was decreased to 1.5% and 1.3% on average in bone marrow and spleen, respectively, compared to 5-6% in wildtype or heterozygotes

• defective receptor editing in B cells showing the presence of unedited DNA-reactive B cells in the periphery

decreased immature B cell number ( J:84040 )

• number of immature B cells (IgM^high IgD^low) is slightly lower

decreased transitional stage B cell number ( J:143854 )

• decreased T2 B cells

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit reduced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• at the T1 to T2 stage

decreased mature B cell number ( J:84040 )

• numbers of mature B cells (IgM^low IgD^high) are severely reduced in the spleens

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal pre-B cell morphology ( J:84040 )

• dominating large pre-B cells are less cycling

decreased IgG3 level ( J:143854 )

decreased IgM level ( J:143854 )

immune system

abnormal B cell apoptosis ( J:143854 )

• spontaneous apoptosis is not inhibited by the addition of BAFF unlike in wild-type cells

increased B cell apoptosis ( J:143854 )

abnormal B cell differentiation ( J:84040 )

• B cell development is partially arrested at the B220+ pre-BCR+ (putative large pre-B) cell stage

abnormal immature B cell morphology ( J:94297 )

• proportion of lamda + B cells was decreased to 1.5% and 1.3% on average in bone marrow and spleen, respectively, compared to 5-6% in wildtype or heterozygotes

• defective receptor editing in B cells showing the presence of unedited DNA-reactive B cells in the periphery

decreased immature B cell number ( J:84040 )

• number of immature B cells (IgM^high IgD^low) is slightly lower

decreased transitional stage B cell number ( J:143854 )

• decreased T2 B cells

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit reduced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• at the T1 to T2 stage

decreased mature B cell number ( J:84040 )

• numbers of mature B cells (IgM^low IgD^high) are severely reduced in the spleens

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal pre-B cell morphology ( J:84040 )

• dominating large pre-B cells are less cycling

decreased IgG3 level ( J:143854 )

decreased IgM level ( J:143854 )

increased anti-double stranded DNA antibody level ( J:94297 )

• four times as much anti-dsDNA IgG antibody was produced upon DNA immunization than in wild-type or heterozygotes

cellular

abnormal B cell apoptosis ( J:143854 )

• spontaneous apoptosis is not inhibited by the addition of BAFF unlike in wild-type cells

increased B cell apoptosis ( J:143854 )

Genotype
MGI:2665858

cx2

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit; Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * NZB

neoplasm

increased leukemia incidence ( J:84040 )

• pre-B cell leukemia

hematopoietic system

enlarged spleen ( J:84040 )

absent immature B cells ( J:84040 )

• immature B cells are absent in the spleen

arrested B cell differentiation ( J:84040 )

• complete arrest at the pre-B cell transition

absent mature B cells ( J:84040 )

• mature B cells are absent in the spleen

abnormal pre-B cell morphology ( J:84040 )

• dominating large pre-B cells are noncycling

decreased pre-B cell number ( J:84040 )

• very few pre-B cells (B220^dull)

immune system

enlarged spleen ( J:84040 )

absent immature B cells ( J:84040 )

• immature B cells are absent in the spleen

arrested B cell differentiation ( J:84040 )

• complete arrest at the pre-B cell transition

absent mature B cells ( J:84040 )

• mature B cells are absent in the spleen

abnormal pre-B cell morphology ( J:84040 )

• dominating large pre-B cells are noncycling

decreased pre-B cell number ( J:84040 )

• very few pre-B cells (B220^dull)

enlarged lymph nodes ( J:84040 )

growth/size/body

enlarged spleen ( J:84040 )

Genotype
MGI:3841693

cx3

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit; Prkcd^tm1Kin/Prkcd^tm1Kin
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * NZB

immune system

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit moderately enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• as in Blnk^tm1Dkit homozygotes

decreased B-1 B cell number ( J:143854 )

• in the peritoneal cavity

decreased B-2 B cell number ( J:143854 )

• in the peritoneal cavity

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG1 level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG2b level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG3 level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

increased IgM level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

hematopoietic system

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit moderately enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• as in Blnk^tm1Dkit homozygotes

decreased B-1 B cell number ( J:143854 )

• in the peritoneal cavity

decreased B-2 B cell number ( J:143854 )

• in the peritoneal cavity

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG1 level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG2b level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG3 level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

increased IgM level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

cellular

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies

Genotype
MGI:3513975

cx4

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit; Igh-J^{tm1(3H9-VDJ)Mwg}/Igh-J⁺
• Genetic Background: involves: C57BL/6 * NZB

hematopoietic system

abnormal immature B cell morphology ( J:94297 )

• impaired editing of anti-DNA Ag receptors in splenic immature B cells

immune system

abnormal immature B cell morphology ( J:94297 )

• impaired editing of anti-DNA Ag receptors in splenic immature B cells

Genotype
MGI:3513981

cx5

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit; Igh-J^{tm1(3H9-VDJ)Mwg}/Igh-J⁺; Igk-J^tm1Mwg/Igk-J⁺
• Genetic Background: involves: C57BL/6 * NZB

hematopoietic system

abnormal immature B cell morphology ( J:94297 )

• decreased number of B cells in bone marrow and spleen

• receptor editing of dsDNA binding BCR replacing kappa chain with lamda was hampered resulting in suppression of lambda + B cells to 5% (compared to 22% in cells with wild-type Blnk)

immune system

abnormal immature B cell morphology ( J:94297 )

• decreased number of B cells in bone marrow and spleen

• receptor editing of dsDNA binding BCR replacing kappa chain with lamda was hampered resulting in suppression of lambda + B cells to 5% (compared to 22% in cells with wild-type Blnk)