Analysis Tools
cardiovascular system
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• at day 3 after wounding, mutants show impaired wound vascularization of the granulation tissues with a thin granulation tissue layer at the wound edge
• by day 7, the wound bed is completely filled with granulation tissue in the dermis of all genotypes; however, the granulation tissue in the wounds of mutant mice appears less vascularized
• at 6 days after wounding, the average vessel size and total vessel area in the wounds of mutant mice are significantly reduced relative to wild-type
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cellular
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• homozygous mutant fibroblasts display a reduced rate of migration in an in vitro wound healing model; no differences are noted between wild-type and heterozygous fibroblasts
• notably, neither FGF2 nor EGF have any effect on the rate of cell migration in these assays
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homeostasis/metabolism
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• excisional skin wounds of 10-week-old wild-type females are reduced by >50% within 2 days and up to 75% within 4 days after wounding; in contrast, mutants exhibit a delay in the rate of wound healing between days 3 and 6 after skin wounding
• by day 9, all mice have closed their wounds to the same extent (~80%); complete wound closure is achieved by day 12
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• at day 3 after skin wounding, mutant mice show a failure of wound contraction
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