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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Casp3tm1Flv
targeted mutation 1, Richard Flavell
MGI:2158753
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Casp3tm1Flv/Casp3tm1Flv B6.129S1-Casp3tm1Flv MGI:3603537
hm2
Casp3tm1Flv/Casp3tm1Flv B6N.129S1-Casp3tm1Flv/J MGI:5446051
hm3
Casp3tm1Flv/Casp3tm1Flv involves: 129S1/Sv * C57BL/6 MGI:2684625
ht4
Casp3tm1Flv/Casp3+ B6.129S1-Casp3tm1Flv MGI:3604111
ht5
Casp3tm1Flv/Casp3+ involves: 129S1/Sv * C57BL/6J MGI:3604024
cx6
Casp3tm1Flv/Casp3tm1Flv
Casp7tm1Flv/Casp7tm1Flv
B6.129S-Casp3tm1Flv Casp7tm1Flv MGI:3810971
cx7
Casp3tm1Flv/Casp3tm1Flv
Casp7tm1Flv/Casp7+
B6.129S-Casp3tm1Flv Casp7tm1Flv MGI:3810970
cx8
Casp3tm1Flv/Casp3tm1Flv
Pde6brd1/Pde6brd1
involves: 129S1 * C3H/FeJ * C57BL/6J MGI:3604192
cx9
Casp3tm1Flv/Casp3tm1Flv
Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli/0
involves: 129S1/Sv * C57BL/6 * CBA MGI:5008743
cx10
Casp3tm1Flv/Casp3tm1Flv
Casp6tm1Flv/Casp6tm1Flv
involves: 129S1/Sv * C57BL/6J MGI:3603541


Genotype
MGI:3603537
hm1
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Genetic
Background
B6.129S1-Casp3tm1Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
(J:70205)
• Background Sensitivity: normal fertility and viable for more than 6 months (J:101037)
• normal ovarian architecture with normal numbers of nonatretic primordial, primary, and small preantral follicles (J:101037)
• normal oocyte apoptosis (J:101037)
• shrunken and disorganized atretic follicles lacking blood cell infiltration
• granulosa cells of atretic follicles are not eliminated by apoptosis and when cultured in the absence of serum fail to complete nuclear fragmentation after initial DNA condensation

vision/eye
N
• the size of the lens organelle free zone is normal
• slight reduction in lysis of fluorogenic substrate DEVD-AMC in both the core and cortex of the lens
• the epithelium at the anterior lens pole is multilayered and disorganized and the lens capsule above the opaque region has irregular folds
• marked cataracts on the optic acis due to accumulation of epithelial cells at the anterior pole of the lens evident by the end of the first postnatal week, mature by 3 weeks of age, but most 6 month to 2 year old homozygotes no longer have cataracts

hearing/vestibular/ear
• the greater epithelial ridge persists at 2 weeks of age throughout all turns of the cochlea
• the tunnel of Corti and other pericellular spaces are collapsed at 5 weeks of age
• hyperplasia and stratified arrangement of border cells throughout all turns of the cochlea at 2 and 5 weeks of age
• at 2 weeks of age there is fusion of stereocilia and at 5 weeks of age all outer hair cell stereocilia are fused at the tips to an unidentified V shaped object
• at 5 weeks of age degeneration of inner hair cells is apparent with only 1 row of inner hair cells present in the apical to middle turn
• at 5 weeks of age degeneration of outer hair cells is apparent with only 3 rows of outer hair cells present in the apical to middle turn
• ABR analysis at 2 weeks of age shows atypical waves at all frequencies with sound pressures above 70 dB
• at 5 weeks of age no significant waves are detected even at 100 dB

nervous system
• at 2 weeks of age there is fusion of stereocilia and at 5 weeks of age all outer hair cell stereocilia are fused at the tips to an unidentified V shaped object
• at 5 weeks of age degeneration of inner hair cells is apparent with only 1 row of inner hair cells present in the apical to middle turn
• at 5 weeks of age degeneration of outer hair cells is apparent with only 3 rows of outer hair cells present in the apical to middle turn
• at 5 weeks of age neurons are lost from the basal spiral ganglion

endocrine/exocrine glands
• shrunken and disorganized atretic follicles lacking blood cell infiltration
• granulosa cells of atretic follicles are not eliminated by apoptosis and when cultured in the absence of serum fail to complete nuclear fragmentation after initial DNA condensation

cellular
• hemorrhagic response and destruction of the hepatic enothelium and subsequent death in response to injection with anti-Fas antibody Jo2 are delayed at least 12 hours relative to that in wildtype mice and the apoptotic pathway aberrently involves activation, but not altered expression, of caspases 2, 6, and 7
• following UV irradiation and staurosporine treatment, mouse embryonic fibroblasts (MEFs) exhibit increased survival compared to wild type MEFs but not as much as Casp7tm1Flv MEFs
• mouse embryonic fibroblasts treated to initiate apoptosis fail to exhibit DNA fragmentation




Genotype
MGI:5446051
hm2
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Genetic
Background
B6N.129S1-Casp3tm1Flv/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• in mice treated with doxorubicin
• mice exhibit decreased sunburn cells with reduced apoptotic keratinocytes generated by UV-B in the skin compared with wild-type mice
• in the cardiomyocytes of mice treated with doxorubicin
• mice exhibit decreased sunburn cells with reduced apoptotic keratinocytes generated by UV-B in the skin compared with wild-type mice
• mice exposed to UV-B radiation exhibit increased keratinocytes undergoing necrosis-like death compared with wild-type mice
• mice exposed to UV-B radiation exhibit increased cell death (apoptotic and necrosis-like combined) compared with wild-type mice

cardiovascular system
• in mice treated with doxorubicin

integument
• mice exposed to UV-B radiation exhibit increased keratinocytes undergoing necrosis-like death compared with wild-type mice
• mice exhibit decreased sunburn cells with reduced apoptotic keratinocytes generated by UV-B in the skin compared with wild-type mice

muscle
• in mice treated with doxorubicin




Genotype
MGI:2684625
hm3
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Skeletal defects in Casp3tm1Flv/Casp3tm1Flv and Casp3tm1Flv/Casp3+ mice

mortality/aging
• Background Sensitivity: animals surviving to birth die between 1-3 weeks of age
• incomplete penetrance with only 7% homozygous offspring from heterozygote x heterozygote breeders

growth/size/body
• smaller than littermates

vision/eye
N
• normal cell number and apoptotic kinetics within the retinal ganglion cell layer and normal width of the nerve fiber and plexiform layers
• protrusions of the neuroepithelium in the retina, causing compression on the lens
• delayed regression of vitreal vasculature
• optic stalk contains fewer pyknotic cells and is much larger than in wild-type
• eyes are 85% to 90% smaller than those of heterozygotes or wild-type
• retinal dysplasia near the optic nerve ranging from absence of retinal pigment epithelium to whorled and folded neuroretina
• in severe cases, the posterior retina is folded and elevated at the optic nerve head
• absence of the retinal pigment epithelium in a region encircling the optic nerve
• delayed apoptotic kinetics result in thickened inner nuclear layer at 5 days of age which resolves after 13 days of age

nervous system
N
• normal cell number and apoptotic kinetics within the retinal ganglion cell layer and normal width of the nerve fiber and plexiform layers
• multiple indentations and ectopic cell masses in the cerebrum mantel
• protrusion of brain tissue from the skull at the frontal bone area
• the germinal layer is thick and mitotically active at P16, at a time when it is normally absent, and spindle-shaped granule cells traverse the molecular layer
• the germinal layer is thick and mitotically active at P16, at a time when it is normally absent
• diencephalic lumen is frequently obstructed at E17
• increase in cell number and density of the brainstem at E12
• ectopic cell masses are found between the cerebral cortex, the hippocampus and the striatum
• thicker diencephalic wall with supernumerary cell masses is seen at E12
• thickness of the internal granular layer is increased
• numerous spindle-shaped granule cells are seen traversing the molecular layer

skeleton
• open sagittal suture of anterior frontal bone at 7 weeks of age
• either completely open sagittal suture or incomplete closure at 7 weeks of age
• osteoclast activity is reduced, most likely due to dysfunctional preosteoblastic cells
• skeleton is smaller than normal at 1 week of age
• reduced trabecular thickness
• reduced bone volume to tissue volume ratio
• evident at E14.5 in skull, metacarpi, phalanges, and sternum and at E17.5 in interparietal bones and metatarsi

craniofacial
• open sagittal suture of anterior frontal bone at 7 weeks of age
• either completely open sagittal suture or incomplete closure at 7 weeks of age

cardiovascular system
• delayed regression of vitreal vasculature, however the heart is histologically normal

pigmentation
• absence of the retinal pigment epithelium in a region encircling the optic nerve

cellular
N
• normal phosphatidyl serine flip-flop in thymocytes treated with dexamethasone (J:59875)
• E12.5 primary telencephalic cultures undergo normal cell death in response to AraC treatment (J:66953)
• pyknotic clusters at sites of major morphogenetic change during brain development are not observed, indicating a decrease in apoptosis in the brain
• however, thymocytes show normal susceptibility to apoptosis

hematopoietic system
• fewer total thymocytes at 2 weeks of age, however the spleen is histologically normal
• osteoclast activity is reduced, most likely due to dysfunctional preosteoblastic cells

immune system
N
• normal thymocyte expression patterns of CD4, CD8, CD25, CD69, and heat stable antigen
• fewer total thymocytes at 2 weeks of age, however the spleen is histologically normal
• osteoclast activity is reduced, most likely due to dysfunctional preosteoblastic cells
• increased replicative senescence in bone marrow stromal stem cells from attenuated cell cycle

renal/urinary system
N
• kidney is histologically normal

respiratory system
N
• lung is histologically normal

reproductive system
N
• testis is histologically normal

endocrine/exocrine glands
• fewer total thymocytes at 2 weeks of age, however the spleen is histologically normal




Genotype
MGI:3604111
ht4
Allelic
Composition
Casp3tm1Flv/Casp3+
Genetic
Background
B6.129S1-Casp3tm1Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hearing/vestibular/ear
N
• hearing and cochlear morphology normal




Genotype
MGI:3604024
ht5
Allelic
Composition
Casp3tm1Flv/Casp3+
Genetic
Background
involves: 129S1/Sv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Skeletal defects in Casp3tm1Flv/Casp3tm1Flv and Casp3tm1Flv/Casp3+ mice

craniofacial
• incomplete closure of sagittal suture of anterior frontal bone at 7 weeks of age

skeleton
• incomplete closure of sagittal suture of anterior frontal bone at 7 weeks of age
• decreased bone mineral density with age with onset beginning at 3 weeks of age
• reduced bone marrow density
• impaired osteogenic differentiation
• reduced trabecular thickness at 3 weeks of age




Genotype
MGI:3810971
cx6
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Casp7tm1Flv/Casp7tm1Flv
Genetic
Background
B6.129S-Casp3tm1Flv Casp7tm1Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
Casp7tm1Flv mutation (1 available); any Casp7 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• though present in Mendelian ratios at E20, mice die rapidly after birth
• mice die rapidly after birth and by P10 to P14 no mice are alive

cellular
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased
• however, thymocytes are still sensitive to death receptor mediated apoptosis
• mouse embryonic fibroblasts (MEFs) subjected to UV irradiation and staurosporine fail to undergo apoptosis
• MEFs exhibit decreased apoptosis following stimulation of the death receptor pathway
• following induction of apoptosis, MEFs fail to exhibit a loss of mitochondrial membrane potential
• mouse embryonic fibroblasts (MEFs) subjected to UV irradiation and staurosporine fail to exhibit evidence of normal apoptosis 24hrs after treatment unlike in wild type MEFs

cardiovascular system
• at E20, the ventricular wall is disorganized and noncompacted

nervous system
• in 10% of mice

immune system
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased
• however, thymocytes are still sensitive to death receptor mediated apoptosis

hematopoietic system
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased
• however, thymocytes are still sensitive to death receptor mediated apoptosis

endocrine/exocrine glands
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased
• however, thymocytes are still sensitive to death receptor mediated apoptosis




Genotype
MGI:3810970
cx7
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Casp7tm1Flv/Casp7+
Genetic
Background
B6.129S-Casp3tm1Flv Casp7tm1Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
Casp7tm1Flv mutation (1 available); any Casp7 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased although not as much as in Casp3tm1Flv Casp7tm1Flv homozygous T cells
• apoptosis is decreased in mouse embryonic fibroblasts (MEFs) subjected to UV irradiation and staurosporine comapred to in wild-type MEFs
• MEFs exhibit decreased apoptosis following stimulation of the death receptor pathway that is intermediate to the response observed in Casp3tm1Flv Casp7tm1Flv homozygous MEFs
• mouse embryonic fibroblasts (MEFs) subjected to UV irradiation and staurosporine exhibit distorted chromosome condensation and nuclear fragmentation is absent unlike in similarly treated MEFs

immune system
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased although not as much as in Casp3tm1Flv Casp7tm1Flv homozygous T cells

hematopoietic system
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased although not as much as in Casp3tm1Flv Casp7tm1Flv homozygous T cells

endocrine/exocrine glands
• following injection of fetal liver cells into a RAG-deficient background, thymocyte apoptosis induced by mitochondrial pathways is decreased although not as much as in Casp3tm1Flv Casp7tm1Flv homozygous T cells




Genotype
MGI:3604192
cx8
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Pde6brd1/Pde6brd1
Genetic
Background
involves: 129S1 * C3H/FeJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
Pde6brd1 mutation (41 available); any Pde6b mutation (101 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• loss of 60% to 90% of rod population despite the absence of CASP3
• outer nuclear layer slightly thicker than with normal CASP3 expression

cellular
• slight reduction in rate of loss of photoreceptors relative to rd1 homozygotes with normal CASP3 expression

nervous system
• loss of 60% to 90% of rod population despite the absence of CASP3




Genotype
MGI:5008743
cx9
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli/0
Genetic
Background
involves: 129S1/Sv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• following treatment with doxycycline but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• doxycycline-treated mice exhibit increased lung volume compared to in wild-type mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• following treatment with doxycycline but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice

immune system
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice

cellular
• in lung cells following treatment with doxycycline but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice

hematopoietic system
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice
• in the bronchoalveolar lavage of doxycycline-treated mice but not as much as in Tg(Scgb1a1-rtTA,tetO-Ifng)14Eli mice




Genotype
MGI:3603541
cx10
Allelic
Composition
Casp3tm1Flv/Casp3tm1Flv
Casp6tm1Flv/Casp6tm1Flv
Genetic
Background
involves: 129S1/Sv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Casp3tm1Flv mutation (1 available); any Casp3 mutation (26 available)
Casp6tm1Flv mutation (1 available); any Casp6 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• slightly smaller than normal

vision/eye
N
• normal tissue organization in the equatorial lens and normal denucleation process in lens
• anterior polar cataracts are more pronounced than in mice disrupted for only caspase 3
• incomplete penetrence, occurs in less than half the mice
• incomplete penetrence





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last database update
10/19/2021
MGI 6.17
The Jackson Laboratory