All Phenotypes Adora2a<tm1Jfc> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adora2a^tm1Jfc/Adora2a^tm1Jfc	B6.129S4-Adora2a^tm1Jfc	MGI:5473699
hm2	Adora2a^tm1Jfc/Adora2a^tm1Jfc	involves: 129 * C57BL/6	MGI:3625471
hm3	Adora2a^tm1Jfc/Adora2a^tm1Jfc	involves: 129S4/SvJae * 129S6/SvEvTac	MGI:3622795
hm4	Adora2a^tm1Jfc/Adora2a^tm1Jfc	involves: 129S4/SvJae * C57BL/6	MGI:3622794
cn5	Adk^tm2Bois/Adk^tm2Bois Adora2a^tm1Jfc/Adora2a^tm1Jfc Tg(Nes-cre)1Kln/0	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5816717
cx6	Adora2a^tm1Jfc/Adora2a^tm1Jfc Drd2^tm1Low/Drd2^tm1Low	involves: 129 * C57BL/6	MGI:3625472

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

abnormal pulmonary artery morphology ( J:194389 )

• cell proliferation is increased in all major cell types across entire pulmonary arterial walls, including smooth muscle, endothelium cells and fibroblasts compared to wild-type mice

• pulmonary arteries exhibit swelling and hypertrophy of endothelial and smooth muscle cells, with abundance of cytoplasm and an increase in intracytoplasmic vesicles

• pulmonary artery endothelium contains more Weibel-Palade bodies than in wild-type, indicating activation of endothelial cells

• cytoplasm of pulmonary artery smooth muscle cells contains numerous filaments and dense bodies, indicating activation of smooth muscle cells

• mutants exhibit enhanced hyperplasia of pulmonary artery fibroblasts, as indicated by an increase in fibroblasts seen outside the external elastic laminae and more clustered collagaen fibers deposition in adventitia pulmonary arterial walls

abnormal lung vasculature morphology ( J:194389 )

• mutants exhibit hypertrophy of pulmonary resistance vessels with increased wall thickness and area

• mice exhibit evidence of pulmonary vascular remodeling, showing fibroblast, smooth muscle and endothelium cell hypertrophy

• however, mutants do not exhibit pulmonary edema, lung inflammation, fibrosis or thickening of the alveolar septa

vascular smooth muscle hypertrophy ( J:194389 )

• pulmonary artery wall exhibits increased smooth muscle cell hypertorphy

heart right ventricle hypertrophy ( J:194389 )

• the mean Fulton index (ratio of right ventricle over left ventricle plus septum) is higher in mutants than wild-type mice at 14-16 weeks of age, indicating hypertrophic right ventricles

increased right ventricle systolic pressure ( J:194389 )

• mice exhibit a 44.8% increase in right ventricular systolic pressure at 14-16 weeks of age

• however, mean systolic blood pressure and heart rate are normal

• following chronic exposure to hypoxia, mutants show an exacerbated elevation in right ventricular systolic pressure

pulmonary hypertension ( J:194389 )

• mice develop pulmonary arterial hypertension without affecting systemic circulation or heart rate

• following chronic exposure to hypoxia, mutants show exacerbated elevation in right ventricular systolic pressure, hypertrophy of pulmonary resistance vessels, and increased cell proliferation in pulmonary resistance vessels compared to wild-type mice, indicating a further increase in pulmonary hypertension under hypoxic conditions

• however, mutants do not show any features of hypertensive nephropathy

muscle

vascular smooth muscle hypertrophy ( J:194389 )

• pulmonary artery wall exhibits increased smooth muscle cell hypertorphy

heart right ventricle hypertrophy ( J:194389 )

• the mean Fulton index (ratio of right ventricle over left ventricle plus septum) is higher in mutants than wild-type mice at 14-16 weeks of age, indicating hypertrophic right ventricles

respiratory system

abnormal lung vasculature morphology ( J:194389 )

• mutants exhibit hypertrophy of pulmonary resistance vessels with increased wall thickness and area

• mice exhibit evidence of pulmonary vascular remodeling, showing fibroblast, smooth muscle and endothelium cell hypertrophy

• however, mutants do not exhibit pulmonary edema, lung inflammation, fibrosis or thickening of the alveolar septa

growth/size/body

heart right ventricle hypertrophy ( J:194389 )

• the mean Fulton index (ratio of right ventricle over left ventricle plus septum) is higher in mutants than wild-type mice at 14-16 weeks of age, indicating hypertrophic right ventricles

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
primary pulmonary hypertension		DOID:14557	OMIM:178600 OMIM:265400 OMIM:615342 OMIM:615343 OMIM:615344	J:194389

Allelic Composition	Adora2a^tm1Jfc/Adora2a^tm1Jfc
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adora2a^tm1Jfc mutation (2 available); any Adora2a mutation (36 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

abnormal locomotor activation ( J:102700 )

• mutants do not show lower levels of locomotor stimulation in response to a Grm5 antagonist, MPEP, whereas compared to wild-type controls

Allelic Composition	Adora2a^tm1Jfc/Adora2a^tm1Jfc
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adora2a^tm1Jfc mutation (2 available); any Adora2a mutation (36 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

impaired behavioral response to addictive substance ( J:106215 , J:107988 )

• homozygotes do not display enhanced locomotor activation (over activation seen after the first exposure) after repeated administration of amphetamines (J:106215)

• however, locomotor responses to dopamine receptor D1A and dopamine receptor 2 agonists is similar to wild-type mice (J:106215)

• single exposure amphetamine stimulated increases in locomotor activity are reduced compared to wild-type mice (J:107988)

nervous system

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

• after middle cerebral arterial occlusion total infarct volume is reduced by 30% compared to wild-type mice

• after middle cerebral arterial occlusion infarct volume in the cerebral cortex and striatum are reduced by 33% and 27%, respectively

cardiovascular system

cardiovascular system phenotype ( J:73594 )

N	• basal mean arterial blood pressure, absolute blood flow, and cortical cerebral blood flow before, during. and after middle cerebral arterial occlusion is similar to wild-type mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:106215 )

• homozygotes do not display increased expression of dynorphin mRNA in the striatum after repeated administration of amphetamines

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

• after middle cerebral arterial occlusion total infarct volume is reduced by 30% compared to wild-type mice

• after middle cerebral arterial occlusion infarct volume in the cerebral cortex and striatum are reduced by 33% and 27%, respectively

Allelic Composition	Adora2a^tm1Jfc/Adora2a^tm1Jfc
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adora2a^tm1Jfc mutation (2 available); any Adora2a mutation (36 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

impaired behavioral response to addictive substance ( J:99612 , J:107988 )

• no significant increase in wakefulness is seen in homozygotes after injection of 15 mg/kg, 10 mg/kg, or 5 mg/kg caffeine, unlike wild-type mice (J:99612)

• however, baseline circadian sleep-wake profiles are identical to wild-type (J:99612)

• single exposure amphetamine or cocaine stimulated increases in locomotor activity are reduced compared to wild-type mice (J:107988)

• however, amphetamine stimulated increases in fine movement are not significantly different from wild-type (J:107988)

decreased locomotor activity ( J:73594 , J:107988 )

• reduced spontaneous activity most prominently in the dark cycle; however normal locomotor circadian rhythms are seen (J:107988)

homeostasis/metabolism

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

• after middle cerebral arterial occlusion total infarct volume is reduced by 26% using hematoxylin and eosin staining or by 77% using TTC staining (marker of intact cellular metabolism) compared to wild-type mice

nervous system

nervous system phenotype ( J:73594 , J:107988 )

N	• morphology of cortex and striatum appears normal (J:73594) • dopaminergic innervation is normal (J:107988)

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

cardiovascular system

cardiovascular system phenotype ( J:73594 )

N	• basal mean arterial blood pressure, absolute blood flow, and cortical cerebral blood flow before, during. and after middle cerebral arterial occlusion is similar to wild-type mice

vision/eye

vision/eye phenotype ( J:164077 )

N	• normal growth in the anterior segment and normal pupil size

abnormal sclera morphology ( J:164077 )

• sclera contains denser collagen fibrils with reduced diameter

myopia ( J:164077 )

• mice develop greater myopia than wild-type mice, that is associated with increases in vitreous chamber depth and axial length from P28 to P56

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
myopia		DOID:11830	OMIM:160700 OMIM:255500 OMIM:300613 OMIM:310460 OMIM:603221 OMIM:608367 OMIM:608474 OMIM:608908 OMIM:609256 OMIM:609257 OMIM:609258 OMIM:609259 OMIM:609994 OMIM:609995 OMIM:610320 OMIM:612554 OMIM:612717 OMIM:613959 OMIM:613969 OMIM:614166 OMIM:614167 OMIM:615420 OMIM:615431 OMIM:615946	J:164077

Allelic Composition	Adk^tm2Bois/Adk^tm2Bois Adora2a^tm1Jfc/Adora2a^tm1Jfc Tg(Nes-cre)1Kln/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adk^tm2Bois mutation (0 available); any Adk mutation (50 available)
Adora2a^tm1Jfc mutation (2 available); any Adora2a mutation (36 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased susceptibility to induction of seizure by inducing agent ( J:237189 )

• mice exhibit a resistance to stress-induced (placement in novel environment) seizures

behavior/neurological

behavior/neurological phenotype ( J:237189 )

N	• mice exhibit normal associative learning and contextual learning

decreased susceptibility to induction of seizure by inducing agent ( J:237189 )

• mice exhibit a resistance to stress-induced (placement in novel environment) seizures

Allelic Composition	Adora2a^tm1Jfc/Adora2a^tm1Jfc Drd2^tm1Low/Drd2^tm1Low
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adora2a^tm1Jfc mutation (2 available); any Adora2a mutation (36 available)
Drd2^tm1Low mutation (1 available); any Drd2 mutation (70 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

abnormal locomotor activation ( J:102700 )

• mutants do not show lower levels of locomotor stimulation in response to a Grm5 antagonist, MPEP, whereas compared to wild-type controls

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23