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Allele Symbol Allele Name Allele ID |
Grin1tm2Stl targeted mutation 2, Susumu Tonegawa MGI:1928279 |
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| Summary |
9 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• thalamocortical afferents corresponding to large whiskers form patterns and display critical period plasticity but their pattering is not as well defined as in controls
• thalamocortical patterns corresponding to sinus hairs and digits are mostly absent
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• dendritic fields of spiny stellate cells do not orient toward thalamocortical axon terminal patches, instead they radiate in all directions covering larger territories, exhibiting profuse branching with increased spine density
• cortex thalamocortical axons form smaller patches and individual axon terminal branching is not as well developed as in controls
• septal areas between thalamocortical axon patches are enlarged
• decreased total axonal length, number of branches (by about 50%) and lateral:vertical field span ratio of thalamocortical axons
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• barrels and barrel boundaries do not develop even at sites where thalamocortical afferents cluster
(J:64064)
• layer IV cells of the somatosensory cortex fail to segregate into barrels
(J:80900)
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• barrel cortex lacks NMDA receptor-mediated excitation
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• average body weight was about 70% of that of controls at P7
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• low growth rate
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• diphtheria-treated mice bilaterally injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus
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• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• impaired hippocampus-dependent, but not hippocampus-independent, fear memory that can be rescued with enriched training
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• impaired olfactory-discrimination memory that can be rescued with enriched training
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• deficit in novel object recognition memory that can be partially rescued with enriched training
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• impaired spatial memory in the hidden-platform Morris water maze test, with some progressive improvement in escape latencies but unimpaired nonspatial learning
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• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors
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• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors, however postsynaptic AMPA receptors as well as presynaptic terminals operate normally
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• lack long term potentiation in the CA1 synapses
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• consolidation and storage of long-term nondeclarative taste memories is impaired in these mice when given doxycycline prior to or within three weeks of training
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• mice given doxycycline have impairment of conditioned taste aversion
• mice are trained to avoid saccharin-containing water by being given a LiCl injection after water consumption
• control mice choose unsweetened water about 83.58% of the time 30 days after training
• mutant mice given doxycycline prior to training only choose unsweetened water 32.24% of the time
• mutant mice given doxycycline 2 weeks after training choose unsweetened water 25.63% of the time
• mutant mice given doxycycline 3 weeks to 4 weeks after training choose unsweetened water 27.99% of the time
• mutant mice given doxycycline 4 weeks after training choose unsweetened water at the same frequency as controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• population responses recordings from the stimulated perforant path in the dentate hilus are not different from controls at 20-24 weeks
• paired-pulse facilitation is normal
• long term potentiation in area CA1 after Schaffer commissural input stimulation in CA3
• hippocampal memory and discrimination is normal in mutants (measured by acquisition of contextual fear conditioning)
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• theta-burst stimulation of the perforant path (PP) did not evoke potentiation (plasticity) of synapses of the PP-GC (dentate gyrus granule cells)
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| N |
• open field activity is not different from controls
• no differences are seen in feeding behavior or body weights compared to controls
• spatial learning in Morris water maze test is normal
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• mice show impaired acquisition of a discrimination task compared to controls; deficit is limited to early days of training, but is comparable to controls after extended training
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• adult mice (25-29 weeks of age) do not show behavioral deficits and display normal social recognition
• mice at more than 32 weeks of age do not deficits in nest building behavior
• mice do not show anxiety-like behaviors
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| N |
• mice display normal prepulse inhibition of the auditory startle reflex
• no differences in mean firing rates or cross-correlation values of pyramidal neurons are detected relative to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• impaired spatial memory recall after partial cue removal but not under full cue conditions in the Morris water maze and mutants exhibited normal motivation, motor coordination and sensory functions
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• CA1 pyramidal cells showed a significant decrease in complex spike bursting properties (complex spike index and burst spike frequency) when mutants were engaged in open-field foraging
• CA1 cells showed significant reductions in burst spike frequency, place field size and integrated firing rate after partial cue removal, however showed no significant changes when mutants were returned to an environment with all the distal cues present
• decreased firing rate of putative CA1 interneurons
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• the 6-cyano-7-dinitroquinoxalline-2,3-dione (DNQX)-insensitive component of the NMDA current was significantly reduced at the recurrent commissural/associational synapse relative to controls
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• long term potentiation was nearly absent at the commissural/associational (CA)-CA3 synapses in 9/11 mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• deficits in learning, memory and conditioning are seen in doxycycline treated mutants where expression of Grin1 is knocked out and expression of Grin1-GFP in the CA1 region of the hippocampus is blocked but deficits in learning, memory and conditioning are not seen in untreated mutants
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• with doxycycline treatment prior to or immediately after training mutants show significantly fewer freezing responses compared to untreated mutants in a retention test following a fear-conditioning task
(J:77659)
• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in a 1-month retention test following a fear-conditioning task is seen
(J:77659)
• no difference in cued-fear conditioning behavior is seen with doxycycline treatment
(J:77659)
• with doxycycline treatment for 30 days in the seventh month after training mutants show severe deficits in retention of remote contextual fear memory in a 9-month contextual retention test
(J:88689)
• no deficit was seen with doxycycline treatment for 7 days in the seventh month after training
(J:88689)
• no difference in cued-fear conditioning behavior is seen with doxycycline treatment
(J:88689)
• 2 months after stopping doxycycline treatment mutants perform normally in 1-day contextual fear retention, visual memory, open field behavior and rotorod tests
(J:88689)
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• with doxycycline treatment prior to or immediately after training mutants exhibited longer escape latency in a hidden-platform water maze compared to untreated mutants
• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in escape latency is seen
• deficits in spatial learning and memory are also seen in mutants with doxycycline treatment prior to testing in the transfer test compared to untreated mutants
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• with doxycycline treatment excitation postsynaptic potentials are absent in the CA1 hippocampal region
(J:77659)
• no long-term potentiation is observed in doxycycline treated homozygotes
(J:77659)
• with a 5 day doxycycline treatment no long-term potentiation is observed in mutants
(J:88689)
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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