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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Grin1tm2Stl
targeted mutation 2, Susumu Tonegawa
MGI:1928279
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Grin1tm2Stl/Grin1tm2Stl involves: 129S4/SvJae * C57BL/6 MGI:2175051
cn2
Emx1tm1(cre)Ito/Emx1+
Grin1tm1Stl/Grin1tm2Stl
involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6 MGI:3581525
cn3
Agrptm2(DTR)Rpa/Agrp+
Grin1tm2Stl/Grin1tm2Stl
involves: 129S4/SvJae * 129S4/SvJaeSor MGI:5427451
cn4
Grin1tm2Stl/Grin1tm2Stl
Tg(Camk2a-cre)T29-1Stl/0
involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:3581524
cn5
Grin1tm2Stl/Grin1tm2Stl
Tg(Actb-tTA)1Jzt/0
Tg(Camk2a-cre)62Jzt/0
Tg(tetO-Grin1/GFP)1Jzt/0
involves: 129S4/SvJae * C57BL/6 * CBA MGI:3847227
cn6
Grin1tm2Stl/Grin1tm2Stl
Tg(Pomc-cre)1Lowl/0
involves: 129S4/SvJae * C57BL/6 * FVB MGI:4362041
cn7
Grin1tm2Stl/Grin1tm2Stl
Tg(Ppp1r2-cre)4127Nkza/0
involves: 129S4/SvJae * C57BL/6N MGI:4421577
cn8
Grin1tm2Stl/Grin1tm2Stl
Tg(Grik4-cre)G32-4Stl/0
involves: C57BL/6 MGI:3582225
cn9
Grin1tm2Stl/Grin1tm2Stl
Tg(Actb-tTA)1Jzt/0
Tg(Camk2a-cre)T29-1Stl/0
Tg(tetO-Grin1/GFP)1Jzt/0
involves: C57BL/6 * CBA MGI:3038603


Genotype
MGI:2175051
hm1
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype




Genotype
MGI:3581525
cn2
Allelic
Composition
Emx1tm1(cre)Ito/Emx1+
Grin1tm1Stl/Grin1tm2Stl
Genetic
Background
involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Emx1tm1(cre)Ito mutation (1 available); any Emx1 mutation (27 available)
Grin1tm1Stl mutation (0 available); any Grin1 mutation (49 available)
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• thalamocortical afferents corresponding to large whiskers form patterns and display critical period plasticity but their pattering is not as well defined as in controls
• thalamocortical patterns corresponding to sinus hairs and digits are mostly absent
• dendritic fields of spiny stellate cells do not orient toward thalamocortical axon terminal patches, instead they radiate in all directions covering larger territories, exhibiting profuse branching with increased spine density
• cortex thalamocortical axons form smaller patches and individual axon terminal branching is not as well developed as in controls
• septal areas between thalamocortical axon patches are enlarged
• decreased total axonal length, number of branches (by about 50%) and lateral:vertical field span ratio of thalamocortical axons
• barrels and barrel boundaries do not develop even at sites where thalamocortical afferents cluster (J:64064)
• layer IV cells of the somatosensory cortex fail to segregate into barrels (J:80900)
• barrel cortex lacks NMDA receptor-mediated excitation

growth/size/body
• average body weight was about 70% of that of controls at P7
• low growth rate




Genotype
MGI:5427451
cn3
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Grin1tm2Stl/Grin1tm2Stl
Genetic
Background
involves: 129S4/SvJae * 129S4/SvJaeSor
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (16 available)
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• diphtheria-treated mice bilaterally injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus

growth/size/body
• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice




Genotype
MGI:3581524
cn4
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Camk2a-cre)T29-1Stl/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Camk2a-cre)T29-1Stl mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• impaired hippocampus-dependent, but not hippocampus-independent, fear memory that can be rescued with enriched training
• impaired olfactory-discrimination memory that can be rescued with enriched training
• deficit in novel object recognition memory that can be partially rescued with enriched training
• impaired spatial memory in the hidden-platform Morris water maze test, with some progressive improvement in escape latencies but unimpaired nonspatial learning

nervous system
• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors
• lack the slow component of the excitatory postsynaptic current mediated by NMDA receptors, however postsynaptic AMPA receptors as well as presynaptic terminals operate normally
• lack long term potentiation in the CA1 synapses




Genotype
MGI:3847227
cn5
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Actb-tTA)1Jzt/0
Tg(Camk2a-cre)62Jzt/0
Tg(tetO-Grin1/GFP)1Jzt/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Actb-tTA)1Jzt mutation (0 available)
Tg(Camk2a-cre)62Jzt mutation (0 available)
Tg(tetO-Grin1/GFP)1Jzt mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• consolidation and storage of long-term nondeclarative taste memories is impaired in these mice when given doxycycline prior to or within three weeks of training
• mice given doxycycline have impairment of conditioned taste aversion
• mice are trained to avoid saccharin-containing water by being given a LiCl injection after water consumption
• control mice choose unsweetened water about 83.58% of the time 30 days after training
• mutant mice given doxycycline prior to training only choose unsweetened water 32.24% of the time
• mutant mice given doxycycline 2 weeks after training choose unsweetened water 25.63% of the time
• mutant mice given doxycycline 3 weeks to 4 weeks after training choose unsweetened water 27.99% of the time
• mutant mice given doxycycline 4 weeks after training choose unsweetened water at the same frequency as controls




Genotype
MGI:4362041
cn6
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Pomc-cre)1Lowl/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Pomc-cre)1Lowl mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
N
• population responses recordings from the stimulated perforant path in the dentate hilus are not different from controls at 20-24 weeks
• paired-pulse facilitation is normal
• long term potentiation in area CA1 after Schaffer commissural input stimulation in CA3
• hippocampal memory and discrimination is normal in mutants (measured by acquisition of contextual fear conditioning)
• theta-burst stimulation of the perforant path (PP) did not evoke potentiation (plasticity) of synapses of the PP-GC (dentate gyrus granule cells)

behavior/neurological
N
• open field activity is not different from controls
• no differences are seen in feeding behavior or body weights compared to controls
• spatial learning in Morris water maze test is normal
• mice show impaired acquisition of a discrimination task compared to controls; deficit is limited to early days of training, but is comparable to controls after extended training




Genotype
MGI:4421577
cn7
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Ppp1r2-cre)4127Nkza/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Ppp1r2-cre)4127Nkza mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
N
• adult mice (25-29 weeks of age) do not show behavioral deficits and display normal social recognition
• mice at more than 32 weeks of age do not deficits in nest building behavior
• mice do not show anxiety-like behaviors

nervous system
N
• mice display normal prepulse inhibition of the auditory startle reflex
• no differences in mean firing rates or cross-correlation values of pyramidal neurons are detected relative to controls




Genotype
MGI:3582225
cn8
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Grik4-cre)G32-4Stl/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Grik4-cre)G32-4Stl mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• impaired spatial memory recall after partial cue removal but not under full cue conditions in the Morris water maze and mutants exhibited normal motivation, motor coordination and sensory functions

nervous system
• CA1 pyramidal cells showed a significant decrease in complex spike bursting properties (complex spike index and burst spike frequency) when mutants were engaged in open-field foraging
• CA1 cells showed significant reductions in burst spike frequency, place field size and integrated firing rate after partial cue removal, however showed no significant changes when mutants were returned to an environment with all the distal cues present
• decreased firing rate of putative CA1 interneurons
• the 6-cyano-7-dinitroquinoxalline-2,3-dione (DNQX)-insensitive component of the NMDA current was significantly reduced at the recurrent commissural/associational synapse relative to controls
• long term potentiation was nearly absent at the commissural/associational (CA)-CA3 synapses in 9/11 mutants




Genotype
MGI:3038603
cn9
Allelic
Composition
Grin1tm2Stl/Grin1tm2Stl
Tg(Actb-tTA)1Jzt/0
Tg(Camk2a-cre)T29-1Stl/0
Tg(tetO-Grin1/GFP)1Jzt/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grin1tm2Stl mutation (1 available); any Grin1 mutation (49 available)
Tg(Actb-tTA)1Jzt mutation (0 available)
Tg(Camk2a-cre)T29-1Stl mutation (2 available)
Tg(tetO-Grin1/GFP)1Jzt mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• deficits in learning, memory and conditioning are seen in doxycycline treated mutants where expression of Grin1 is knocked out and expression of Grin1-GFP in the CA1 region of the hippocampus is blocked but deficits in learning, memory and conditioning are not seen in untreated mutants
• with doxycycline treatment prior to or immediately after training mutants show significantly fewer freezing responses compared to untreated mutants in a retention test following a fear-conditioning task (J:77659)
• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in a 1-month retention test following a fear-conditioning task is seen (J:77659)
• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:77659)
• with doxycycline treatment for 30 days in the seventh month after training mutants show severe deficits in retention of remote contextual fear memory in a 9-month contextual retention test (J:88689)
• no deficit was seen with doxycycline treatment for 7 days in the seventh month after training (J:88689)
• no difference in cued-fear conditioning behavior is seen with doxycycline treatment (J:88689)
• 2 months after stopping doxycycline treatment mutants perform normally in 1-day contextual fear retention, visual memory, open field behavior and rotorod tests (J:88689)
• with doxycycline treatment prior to or immediately after training mutants exhibited longer escape latency in a hidden-platform water maze compared to untreated mutants
• with doxycycline treatment just prior to testing (investigating the role of Grin1 in memory retrieval) no significant difference in escape latency is seen
• deficits in spatial learning and memory are also seen in mutants with doxycycline treatment prior to testing in the transfer test compared to untreated mutants

nervous system
• with doxycycline treatment excitation postsynaptic potentials are absent in the CA1 hippocampal region (J:77659)
• no long-term potentiation is observed in doxycycline treated homozygotes (J:77659)
• with a 5 day doxycycline treatment no long-term potentiation is observed in mutants (J:88689)





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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory