Phenotypes associated with this allele

• Allele Symbol: Il5^tm1Kopf
• Allele Name: targeted mutation 1, Manfred A Kopf
• Allele ID: MGI:1861948
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il5^tm1Kopf/Il5^tm1Kopf	C57BL/6-Il5^tm1Kopf	MGI:3621655
hm2	Il5^tm1Kopf/Il5^tm1Kopf	C57BL/6-Il5^tm1Kopf/J	MGI:2179966
hm3	Il5^tm1Kopf/Il5^tm1Kopf	either: C57BL/6-Il5^tm1Kopf or (involves: BALB/c * C57BL/6) or (involves: C57BL/6 * CBA)	MGI:3621737

Genotype
MGI:3621655

hm1

• Allelic Composition: Il5^tm1Kopf/Il5^tm1Kopf
• Genetic Background: C57BL/6-Il5^tm1Kopf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

decreased airway responsiveness ( J:30641 )

• airways of aeroallergen-challenged knockout mice lose hyperresponsiveness to methacholine

immune system

increased neutrophil cell number ( J:30641 )

• after aerosolized OVA challenge, knockout mice have increased levels of neutrophils in BALF compared to unchallenged animals

increased lymphocyte cell number ( J:30641 )

• after aerosolized OVA challenge, knockout mice have increased levels of lymphocytes in BALF compared to unchallenged animals but the increase is less pronounced than in wild-type mice

increased interferon-gamma secretion ( J:107418 )

• in mesenteric lymph nodes in culture, there is a marked increase in IFNG production in knockouts compared to wild-type after restimulation with mitogen or SEA

granulomatous inflammation ( J:107418 )

• pulmonary granulomas that form in knockout mice in response to schistosome eggs are almost completely devoid of eosinophils compared to granulomas in wild-type mice; the average size of granulomas in knockout mice are 40% smaller than those of wild-type

• there is a 20-25% increase in numbers of macrophages associated with granulomas in knockouts compared with wild-type; with acute or chronic parasite infection, the numbers of both fibroblasts and macrophages nearly double in knockout animals compared to wild type

liver/biliary system

liver fibrosis ( J:107418 )

• hepatic fibrosis is significantly reduced in knockouts with acute or chronic helminth parasite infection

hematopoietic system

increased neutrophil cell number ( J:30641 )

• after aerosolized OVA challenge, knockout mice have increased levels of neutrophils in BALF compared to unchallenged animals

increased lymphocyte cell number ( J:30641 )

• after aerosolized OVA challenge, knockout mice have increased levels of lymphocytes in BALF compared to unchallenged animals but the increase is less pronounced than in wild-type mice

Genotype
MGI:2179966

hm2

• Allelic Composition: Il5^tm1Kopf/Il5^tm1Kopf
• Genetic Background: C57BL/6-Il5^tm1Kopf/J

immune system

increased eosinophil cell number ( J:30867 )

• knockout mice infected with parasites do not show eosinophil counts above baseline values, while 35-week old controls develop eosinophilia in the blood, bone marrow and peritoneal cavity

hematopoietic system

increased eosinophil cell number ( J:30867 )

• knockout mice infected with parasites do not show eosinophil counts above baseline values, while 35-week old controls develop eosinophilia in the blood, bone marrow and peritoneal cavity

Genotype
MGI:3621737

hm3

• Allelic Composition: Il5^tm1Kopf/Il5^tm1Kopf
• Genetic Background: either: C57BL/6-Il5^tm1Kopf or (involves: BALB/c * C57BL/6) or (involves: C57BL/6 * CBA)

embryo

enlarged placenta ( J:65853 )

• knockout females have a significant increase in placenta size in matings with CBA males and the fetal:placental ratio is reduced

reproductive system

prolonged estrous cycle ( J:65853 )

♀ • duration of oestrus in knockouts is moderately extended (5.6 days versus 5 days in controls)

behavior/neurological

abnormal mating receptivity ( J:65853 )

♀ • Background Sensitivity: duration of mating interval to achieve mating with syngeneic (B6) versus allogeneic (CBA or BALB/c) males is increased

♀ • after correction for male background, knockout females have significantly lower mating intervals than control females

growth/size/body

increased body weight ( J:65853 )

• Background Sensitivity: allogeneic matings produce larger pups than syngeneic pups; in CBA F1 pups, at weaning, female and male weights are increased 9.5 and 10%, while in C57BL/6 F1 pups, female and male weights at 6 weeks are increased by 7.9 and 12.2%

• Il5 deficient pups have an increased weight gain trajectory compared to wild-type;

• knockout pups are significantly larger than wild-type pups from birth to adulthood