Phenotypes associated with this allele
Allelic Composition |
Os/Os
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Genetic Background |
involves: 101 * C3H |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Os mutation
(9 available);
any
Os mutation
(9 available)
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mortality/aging
embryo
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• cells of the developing embryo appear abnormal between the 7th and 8th division with suggestion of mitotic dysfunction
(J:5017)
• the most striking abnormalities are seen by the middle of the 4th day when many embryonic cells contain fragmented, pycnotic chromatin and lack nuclear membrane and nucleolus
(J:5017)
• in culture, blastocysts show rapid degeneration of the inner cell mass compared with control embryos
(J:5768)
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cellular
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• E4.5 embryos have an index nine times that of controls
• more than one third of cells contain mitotic figures
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growth/size/body
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Os mutation
(9 available);
any
Os mutation
(9 available)
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renal/urinary system
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• glomerular volume is increased 2-fold relative to controls at 3 months of age and 3-fold at 5 months of age but the glomerulosclerosis found on the ROP background is absent with only a minimal increase in extracellular matrix at 5 months of age
• the mean cell number per glomerulus is increased 21% relative to controls and the glomerular labeling index is increased 2.6 fold at 3 and 5 months of age
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• only a minimal increase in extracellular matrix is noted at 5 months of age
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• mean glomerular number per kidney is reduced by 50% at 3 months of age
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• 34% reduction in kidney weight compared with controls at 3 months of age
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Allelic Composition |
Os/Os+
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Genetic Background |
involves: 101 * C3H |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Os mutation
(9 available);
any
Os mutation
(9 available)
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embryo
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• retardation of the mesodermal growth in the preaxial area of the footplate of the forelimb buds is found at embryonic day 10 hour 16 and in the hindlimb buds at embryonic day 10 hour 21
• although normal at early embryonic day 11, by embryonic day 11 to 12 there are cytolytic changes, cellular degeneration, in the preaxial part of the footplate mesoderm, leading to coalescence of the second and third digital rudiments
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limbs/digits/tail
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• retardation of the mesodermal growth in the preaxial area of the footplate of the forelimb buds is found at embryonic day 10 hour 16 and in the hindlimb buds at embryonic day 10 hour 21
• although normal at early embryonic day 11, by embryonic day 11 to 12 there are cytolytic changes, cellular degeneration, in the preaxial part of the footplate mesoderm, leading to coalescence of the second and third digital rudiments
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Allelic Composition |
Os/Os+
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Genetic Background |
involves: 101 * C3H * CBA/Gr |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Os mutation
(9 available);
any
Os mutation
(9 available)
|
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mortality/aging
limbs/digits/tail
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• a reduction in the amount of mesenchyme in the preaxial area of the foot plate is observed by E13
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• ulnar end of the hamatum articulates with metacarpal 4, but does not reach metacarpal 5
• metacarpal 5 is in a fixed state of abduction
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• extensive fusions in carpus
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• digit loss arises by fusion of digits 2 and 3, however, digit 2 is often thinner than normal and may vanish without fusion to digit 3
(J:12942)
• digits 2 and 3 are typically involved
(J:13049)
• different digits in the same foot can be both polydactylous and oligodactylous
(J:13049)
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• exhibited in the hindfeet of some animals
• different digits in the same foot can be both polydactylous and oligodactylous
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• hard tissue fusions start at the basal phalanges and spread distally
(J:12942)
• all fusions between metacarpal and metatarsals are secondary
(J:12942)
• all four feet are affected, although the forefeet are less severely affected than the hindfeet
(J:13049)
• syndactylism primarily involves digits 2 and 3
(J:13049)
• nearly all animals exhibit osseous fusions of the bases of metacarpalia or metatarsalia 4 and 5
(J:13049)
• some animals exhibit fusion of metatarsalia 1 and 2
(J:13049)
• most fusions are secondary, only a few of the fusions are primary hard tissue in both embryo and adult
(J:13049)
• in the tarsus, only the cuneiforme 3 and cuboideum fusion is primary
(J:13049)
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• exhibited in some animals
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• long axis of calcaneus and metararsalia are not parallel, as a result hindfeet point outward
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• calcaneus frequently lacks process trochlearis
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• naviculare is narrow as compared to wildtype
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• extensive and varied fusions in tarsus, which includes a solid fusion between talus and calcaneus
• fusion occurs between the naviculare and a composite of cuneiforme 3 and cuboideum in all animals
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• beginning at E11 the preaxial border of the foot plate is flattened, displaying an ovoid rather than circular outline
• blastemata are crowded and small
• interdigital area between digits 2 and 3 is reduced by E13
• in most cases, digit 2 is formed closer to and may fuse to digit 3
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skeleton
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• ulnar end of the hamatum articulates with metacarpal 4, but does not reach metacarpal 5
• metacarpal 5 is in a fixed state of abduction
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• extensive fusions in carpus
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• long axis of calcaneus and metararsalia are not parallel, as a result hindfeet point outward
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• calcaneus frequently lacks process trochlearis
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• naviculare is narrow as compared to wildtype
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• extensive and varied fusions in tarsus, which includes a solid fusion between talus and calcaneus
• fusion occurs between the naviculare and a composite of cuneiforme 3 and cuboideum in all animals
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embryo
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• at E14 projections of digits 2 and 3 at the edge of the foot plate are closer together than wildtype and there is only a single basal phalanx common to both digits
• at E14-5 cuneiforme 3 and cuboideum have fused to a single element and are elongated in a diagonal direction rather than circular
• at E16 metacarpalia 4 and 5 are fused
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• a reduction in the amount of mesenchyme in the preaxial area of the foot plate is observed by E13
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Os mutation
(9 available);
any
Os mutation
(9 available)
|
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renal/urinary system
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• renal mass is reduced by 38% in comparison to wild-type
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• the principal cell type in the collecting duct is hypertrophied, with the greatest degree of hypertrophy in the nephrectomized heterozygotes
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• females at 3 months of age have a 24% reduction in kidney weight but no concomitant change in body or heart weight
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• in nephrectomized heterozygote males, compensatory kidney growth is reduced in comparison to controls
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• hypertrophied nephrons in females at 3 months of age
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• size of glomeruli is slightly increased in left kidney following unilateral nephrectomy as compared to control
(J:3842)
• in females at 3 weeks of age there is a 34% increase in mean cell number per glomerulus compared with controls
(J:28323)
• Background Sensitivity: this mutation on a C57BL/6J background does not show significant glomerular histopathology
(J:102341)
• bone marrow transplant from heterozygotes into wild-type coisognic hosts results in mesangial sclerosis and glomerular hypertrophy
(J:166810)
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• glomerular cross-sectional area in females assessed at 3 months of age is increased by 1.8 fold compared with controls, although the fraction of the cortex occupied by glomeruli remains the same between mutant and control
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• in females at 3 months of age mesangial sclerosis affects all glomeruli and is evenly distributed in the cortical and justaglomerular regions, with increased extracellular matrix including type IV collagen and tenascin, but no inflammatory cells are found
(J:28323)
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• Background Sensitivity: on the ROP background but not the C57BL/6J background at 5 months of age there is severe and diffuse glomerulosclerosis restricted to the mesangial regions, with a large excess of type IV collagen and tenascin in the mesangial areas
(J:32764)
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• midtranverse sections from the left kidney indicate that glomeruli density is reduced by 50% in heterozygotes
(J:3842)
• females assessed at 3 months of age have a 55% reduction in the mean glomerular number per kidney
(J:28323)
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• segments of proximal tubule, especially pars recta, exhibit hypertrophy
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• diameters of the proximal convoluted and straight tubules are increased in size as compared to wild-type
• tubular epithelial cells are hypertrophied in both heterozyote and nephrectomized heterozygotes, however, the magnitude of hypertrophy is increased in unaltered mice
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• rate of urine flow is increased in heterozygotes as compared to controls, however glomerular filtration rate is not affected
• excretion of creatinine, sodium and potassium is similar to control
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homeostasis/metabolism
immune system
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• glomerular cross-sectional area in females assessed at 3 months of age is increased by 1.8 fold compared with controls, although the fraction of the cortex occupied by glomeruli remains the same between mutant and control
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