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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Jak3tm1Ljb
targeted mutation 1, Leslie J Berg
MGI:1857276
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Jak3tm1Ljb/Jak3tm1Ljb B6;129S4-Jak3tm1Ljb/J MGI:3769351
hm2
Jak3tm1Ljb/Jak3tm1Ljb involves: 129S4/SvJae MGI:3769345
hm3
Jak3tm1Ljb/Jak3tm1Ljb involves: 129S4/SvJae * C57BL/6 MGI:2181271
ht4
Jak3tm1Ljb/Jak3+ involves: 129S4/SvJae MGI:3769346
ht5
Jak3M1Pgrs/Jak3tm1Ljb involves: 129S4/SvJae * C57BL/6J * C57BL/10J MGI:5468017
cx6
Jak3tm1Ljb/Jak3tm1Ljb
Rag1tm1Mom/Rag1tm1Mom
involves: 129S4/SvJae * 129S7/SvEvBrd MGI:3769348
cx7
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)2Ljb/0
involves: 129S4/SvJae * C3H * C57BL/6 MGI:3769347
cx8
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)1Ljb/0
involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10 MGI:3769355
cx9
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)2Ljb/0
involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10 MGI:3769356


Genotype
MGI:3769351
hm1
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Genetic
Background
B6;129S4-Jak3tm1Ljb/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• decrease in the proportion and absolute number of CD4+ cells in the spleen
• decrease in the proportion and absolute number of CD8+ cells in the spleen
• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls
• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls
• the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen
• however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls
• reduced numbers of splenocytes
• decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen
• however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls
• in culture, dendritic cells are resistant to cytokine withdrawal-induced apoptosis
• bone marrow-derived dendritic cells overproduce IL12 and IL10
• when bone marrow-derived dendritic cells are used in an adoptive transfer model the proportion of IFNG-producing cells is increased compared to when wild-type cells are used

hematopoietic system
• decrease in the proportion and absolute number of CD4+ cells in the spleen
• decrease in the proportion and absolute number of CD8+ cells in the spleen
• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls
• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls
• the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen
• however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls
• reduced numbers of splenocytes
• decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen
• however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls

cellular
• splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls
• however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls




Genotype
MGI:3769345
hm2
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• increase in the ratio of CD4+ to CD8+ cells
• increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow
• large increase in the number of cells classified as myeloid or premonocytic
• the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found
• both pro-T and pre-T cells have increased CD25 expression
• about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17
• intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells
• most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage
• from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates
• however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates
• lymphopenia is seen in peripheral and bone marrow smears
• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased
• in adult mice, there is about a 20-fold reduction in thymocyte numbers
• at E14 - E15, thymocytes are virtually undetectable
• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates
• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates
• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen
• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage
• significantly higher numbers of both segmented and band neutrophils
• at 6 - 12 months of age in the bone marrow and spleen
• the general architecture of the spleen is severely disrupted
• may be seen in some mice by as early as 4 months of age
• seen in all mice over 5 months of age
• the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+
• numerous megakaryoctes are present
• thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls
• splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation
• CD4+ cells that are present show an activated phenotype (J:56629)
• CD4+ T cells resemble activated or memory T cells (J:128694)
• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent
• widespread infiltration of the lungs, kidneys, and liver with mononuclear cells

hematopoietic system
• increase in the ratio of CD4+ to CD8+ cells
• slight decrease in the number of erythroid progenitors in the bone marrow
• increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow
• large increase in the number of cells classified as myeloid or premonocytic
• the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found
• both pro-T and pre-T cells have increased CD25 expression
• about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17
• intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells
• most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage
• from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates
• however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates
• lymphopenia is seen in peripheral and bone marrow smears
• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased
• in adult mice, there is about a 20-fold reduction in thymocyte numbers
• at E14 - E15, thymocytes are virtually undetectable
• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates
• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates
• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen
• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage
• significantly higher numbers of both segmented and band neutrophils
• at 6 - 12 months of age in the bone marrow and spleen
• the general architecture of the spleen is severely disrupted
• may be seen in some mice by as early as 4 months of age
• seen in all mice over 5 months of age
• the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+
• numerous megakaryoctes are present
• thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls
• splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation
• CD4+ cells that are present show an activated phenotype (J:56629)
• CD4+ T cells resemble activated or memory T cells (J:128694)
• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent

endocrine/exocrine glands
• increase in the ratio of CD4+ to CD8+ cells
• in adult mice, there is about a 20-fold reduction in thymocyte numbers
• at E14 - E15, thymocytes are virtually undetectable
• at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates
• despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates

cellular
• decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent

growth/size/body
• may be seen in some mice by as early as 4 months of age
• seen in all mice over 5 months of age




Genotype
MGI:2181271
hm3
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• total cell numbers range from 0.5 to 10% of wild-type controls
• however, the proportion of different cell types is similar to controls
• decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen
• however, no significant decrease in the number of CD43+ CD45R+ cells is detected
• profound block in B cell development at the pre-B cell stage
• however, total bone marrow cell numbers are similar to wild-type controls
• virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen
• peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells
• total spleen cell numbers range from 10 to 500% of wild-type controls
• spleens contain an increased percentage of Thy1- CD45R- Mac1- cells
• Peyer's patches are almost undetectable
• peripheral lymph nodes are nearly undetectable
• peripheral lymph nodes contain only about 2% the normal number of cells
• the ratio of CD4+ to CD8+ cells is increase with more than 60% of the cells being CD4+ and less than 5% being CD8+
• decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12
• however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls
• decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12
• cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors
• serum IgG levels appear to be lower in mice at 3 months of age
• however, in younger mice serum Ig levels are similar to wild-type
• splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2
• most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells
• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells
• the LPS-induced proliferation response of spleen cells is significantly reduced

hematopoietic system
• total cell numbers range from 0.5 to 10% of wild-type controls
• however, the proportion of different cell types is similar to controls
• decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen
• however, no significant decrease in the number of CD43+ CD45R+ cells is detected
• profound block in B cell development at the pre-B cell stage
• however, total bone marrow cell numbers are similar to wild-type controls
• virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen
• peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells
• total spleen cell numbers range from 10 to 500% of wild-type controls
• spleens contain an increased percentage of Thy1- CD45R- Mac1- cells
• decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12
• however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls
• decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12
• cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors
• serum IgG levels appear to be lower in mice at 3 months of age
• however, in younger mice serum Ig levels are similar to wild-type
• splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2
• most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells
• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells

endocrine/exocrine glands
• total cell numbers range from 0.5 to 10% of wild-type controls
• however, the proportion of different cell types is similar to controls

cellular
• mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells




Genotype
MGI:3769346
ht4
Allelic
Composition
Jak3tm1Ljb/Jak3+
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• slight increase in the number of cells classified as myeloid or premonocytic
• lymphopenia is seen in peripheral and bone marrow smears
• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased
• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen
• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage
• significantly higher numbers of both segmented and band neutrophils
• at 6 - 12 months of age in the bone marrow and spleen

hematopoietic system
• slight increase in the number of cells classified as myeloid or premonocytic
• lymphopenia is seen in peripheral and bone marrow smears
• at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased
• at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen
• these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage
• significantly higher numbers of both segmented and band neutrophils
• at 6 - 12 months of age in the bone marrow and spleen




Genotype
MGI:5468017
ht5
Allelic
Composition
Jak3M1Pgrs/Jak3tm1Ljb
Genetic
Background
involves: 129S4/SvJae * C57BL/6J * C57BL/10J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3M1Pgrs mutation (0 available); any Jak3 mutation (84 available)
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice are resistant lethality associated with cerebral malaria induced by infection with Plasmodium berghei ANKA-parasitized red blood cells compared with C57BL/10J mice

immune system
• mice are resistant lethality associated with cerebral malaria induced by infection with Plasmodium berghei ANKA-parasitized red blood cells compared with C57BL/10J mice




Genotype
MGI:3769348
cx6
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Rag1tm1Mom/Rag1tm1Mom
Genetic
Background
involves: 129S4/SvJae * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (120 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• unlike Jak3 null mice that are wild-type for Rag1, no splenomegaly is seen
• FACs profile of spleens resemble those of mice homozygous null for Rag1 alone

hematopoietic system
• FACs profile of spleens resemble those of mice homozygous null for Rag1 alone




Genotype
MGI:3769347
cx7
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)2Ljb/0
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
Tg(Lck-Jak3)2Ljb mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• unlike in homozygous null mice that do not express the transgene, the numbers of CD4 and CD8 cells in the spleen and thymus are normal
• splenomegaly apparent after 12 weeks of age
• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene
• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene
• CD4+ cells show an activated phenotype

hematopoietic system
• splenomegaly apparent after 12 weeks of age
• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene
• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene
• CD4+ cells show an activated phenotype

growth/size/body
• splenomegaly apparent after 12 weeks of age
• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene




Genotype
MGI:3769355
cx8
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)1Ljb/0
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
Tg(Lck-Jak3)1Ljb mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• unlike null mice without the transgene, thymic cellularity, the ratio of CD4+ to CD8+ cells in the thymus, T cell maturation in the spleen, and the numbers of gamma delta T and NK cells are normal
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced
• absence of CD45R+ IgM+ cells in the bone marrow

hematopoietic system
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced
• absence of CD45R+ IgM+ cells in the bone marrow

cellular
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced




Genotype
MGI:3769356
cx9
Allelic
Composition
Jak3tm1Ljb/Jak3tm1Ljb
Tg(Lck-Jak3)2Ljb/0
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak3tm1Ljb mutation (1 available); any Jak3 mutation (84 available)
Tg(Lck-Jak3)2Ljb mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• unlike null mice without the transgene, thymic cellularity, the ratio of CD4+ to CD8+ cells in the thymus, T cell maturation in the spleen, and the numbers of gamma delta T and NK cells are normal
• absence of CD45R+ IgM+ cells in the bone marrow
• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)
• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases
• this correlates with the decrease in transgene expression in the periphery as mice age
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

hematopoietic system
• absence of CD45R+ IgM+ cells in the bone marrow
• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)
• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases
• this correlates with the decrease in transgene expression in the periphery as mice age
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

cellular
• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced





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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory