Fus^tm2.1Ics
Targeted Allele Detail

Summary

• Symbol: Fus^tm2.1Ics
• Name: fused in sarcoma; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:8280967
• Gene: Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
• Alliance: Fus^tm2.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Altered localization, Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 12 and a second loxP site, an FRT site flanked neomycin resistance gene cassette, a synthetic DNA fragment (consisting of last 254 bp of intron 12, fused exon 13 (ENSMUSE00001235788) and 14 (ENSMUSE00001215589) coding sequences and a STOP cassette (containing 3x SV40 poly(A) signal sequences) were inserted downstream of the gene. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wild-type protein and only after Cre-mediated deletion of the endogenous exons 13-15 will it express protein lacking the NLS sequence that is encoded by exon 15. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fus Mutation: 58 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)