Nrxn1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nrxn1^{tm1d(KOMP)Wtsi}
• Name: neurexin I; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:8161771
• Gene: Nrxn1 Location: Chr17:90341059-91400499 bp, - strand Genetic Position: Chr17, 59.73 cM
• Alliance: Nrxn1^{tm1d(KOMP)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148605
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 90950152 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 90951300. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination deleted critical exon(s) and generated a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrxn1 Mutation: 104 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 1 reference(s)