En2^em1Alj
Endonuclease-mediated Allele Detail

Summary

• Symbol: En2^em1Alj
• Name: engrailed 2; endonuclease-mediated mutation 1, Alexandra L Joyner
• MGI ID: MGI:7789004
• Synonyms: En2-HA
• Gene: En2 Location: Chr5:28370692-28377164 bp, + strand Genetic Position: Chr5, 13.94 cM
• Alliance: En2^em1Alj page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: Using CRISPR technology, a single-stranded oligodeoxynucleotide (ssODN) was designed to insert a linker (GlyLysSerAspSerGlu) and three consecutive HA tags (3xHA) in-frame into the C-terminal of the En2 gene, replacing the endogenous stop codon. (J:101977, J:292768)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En2 Mutation: 105 strains or lines available

References

• Original: J:292768 Willett RT, et al., Cerebellar nuclei excitatory neurons regulate developmental scaling of presynaptic Purkinje cell number and organ growth. Elife. 2019 Nov 19;8:e50617
• All: 2 reference(s)