Cic^em1(DUX4)Dgk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cic^em1(DUX4)Dgk
• Name: capicua transcriptional repressor; endonuclease-mediated mutation 1, David Kirsch
• MGI ID: MGI:7738786
• Synonyms: Ch7CDS
• Gene: Cic Location: Chr7:24967129-24993584 bp, + strand Genetic Position: Chr7, 13.73 cM
• Alliance: Cic^em1(DUX4)Dgk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347765
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Cic^em1(DUX4)Dgk expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DUX4 (100288687)

  Duxf3	(MGI:1921649)
  Duxf4	(MGI:3645498)

  conditional expression of the C terminal domain fused to mouse Cic

• Mutation details: A loxP site was inserted upstream of exon 20 and a loxP site and exon 20 fused to the C terminal domain of human DUX4 were inserted downstream of the polyA signal vis CRISPR/cas9 mediated recombination. In the absence of cre expression this allele expresses the wild-type protein but after cre-mediated recombination a Cic-DUX4 fusion protein is expressed. (J:347765)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cic Mutation: 99 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:347765 Hendrickson PG, et al., Spontaneous expression of the CIC::DUX4 fusion oncoprotein from a conditional allele potently drives sarcoma formation in genetically engineered mice. Oncogene. 2024 Apr;43(16):1223-1230
• All: 1 reference(s)