Saa3^em2Litt
Endonuclease-mediated Allele Detail

Summary

• Symbol: Saa3^em2Litt
• Name: serum amyloid A 3; endonuclease-mediated mutation 2, Dan R Littman
• MGI ID: MGI:7717772
• Synonyms: SAA^TKO
• Gene: Saa3 Location: Chr7:46361422-46365100 bp, - strand Genetic Position: Chr7, 30.51 cM
• Alliance: Saa3^em2Litt page

Mutation origin

• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing is used to introduce premature stop codons (TAG TAA), a frame shift mutation (A), and a HindIII site (AAGCTT) in to exon 2. Recombination was done using mice carrying Del(7Saa1-Saa2)1Itl creating triple knockout mice. (J:296071)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Saa3 Mutation: 22 strains or lines available

References

• Original: J:296071 Lee JY, et al., Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. Cell. 2020 Jan 9;180(1):79-91.e16
• All: 3 reference(s)