Igs2^{tm1.1(CAG-CHRM3*/mCherry)Gida}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1.1(CAG-CHRM3*/mCherry)Gida}
• Name: intergenic site 2; targeted mutation 1.1, Giuseppe d'Agostino
• MGI ID: MGI:7716799
• Synonyms: LSL-hM3Dq-T2A-mCherry
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1.1(CAG-CHRM3*/mCherry)Gida} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342741
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Igs2^{tm1.1(CAG-CHRM3*/mCherry)Gida} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CHRM3 (1131)

  Chrm3

  (MGI:88398)

  modified with p.Y149C and p.A239G to become DREADD version

• Mutation details: A vector containing the following elements was inserted into the three tandem attP sequences the locus previously had been modified with, using phage phiC31: attB site, FRT site flanked STOP cassette, loxP site flanked STOP cassette, CAG promoter, hM3Dq cDNA, sequence coding for T2A self-cleaving peptide, mCherry reporter gene, SV40 poly(A) signal sequences, and second attB site. The hM3Dq sequence is a Gq-coupled human M3 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM3Dq sequence, the wildtype human muscarinic 3 receptor (CHRM3) sequence was modified with two amino acid substitutions (p.Y149C and p.A239G) that abolish receptor affinity for the native ligand acetylcholine (ACh) but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). The FRT site flanked STOP cassette was removed through subsequent Flp-mediated recombination. (J:342741)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:342741 Boutagouga Boudjadja M, et al., Hypothalamic AgRP neurons exert top-down control on systemic TNF-alpha release during endotoxemia. Curr Biol. 2022 Nov 7;32(21):4699-4706.e4
• All: 2 reference(s)