Igk^em3Abtx
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igk^em3Abtx
• Name: immunoglobulin kappa chain complex; endonuclease-mediated mutation 3, Dan Rohrer
• MGI ID: MGI:7715233
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The allele has the mouse immunoglobulin kappa chain complex (Igk) region variable (V), joining (J), MAR/intronic enhancer (iE) and constant (C) segments deleted, then replaced with a 'landing pad'. Specifically, CRISPR/Cas9 genome editing in 129S6/SvEvTac-derived CMTI-1 embryonic stem (ES) cells was used to remove a ~3.2 Mb sequence including the mouse Igk V-J-C segments and replace it with a ~2 kb 'landing pad' comprised of a loxP-flanked neomycin resistance cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 33 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)